Head-to-head comparisons of carbon fiber microelectrode coatings for sensitive and selective neurotransmitter detection by voltammetry.
Head-to-head comparisons of carbon fiber microelectrode coatings for sensitive and selective neurotransmitter detection by voltammetry.
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DOI：
10.1021/ac2011729
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发表时间：
2011-09-01
影响因子：
7.4
通讯作者：
Andrews, Anne M.
中科院分区：
文献类型：
--
作者：
Singh, Yogesh S.;Sawarynski, Lauren E.;Dabiri, Pasha D.;Choi, Wonwoo R.;Andrews, Anne M.
伏安法被广泛用于研究神经传递和其他生物过程，但受到化学选择性差和常用碳纤维微电极（CFMs）污染的限制。我们进行了直接比较的三个关键涂层材料声称赋予电极的选择性和防污性：全氟磺酸，碱水解醋酸纤维素（BCA），和纤连蛋白。我们系统地评估了对一系列电极参数的影响。使用一种方法研究了由于暴露于脑组织而导致的污染，该方法最大限度地减少了动物的使用，同时能够评价具有统计学意义的电极群体。我们发现，BCA是相对抗污垢。此外，可以通过改变水解时间来调整在BCA涂覆的CFMs处的检测，以最小化对灵敏度损失的影响，同时保持抗结垢性。纤连蛋白涂层与涂层和污染后的灵敏度中度损失相关。全氟磺酸增加了多巴胺和去甲肾上腺素的敏感性，但没有血清素，以及预期的选择性阳离子神经递质阴离子代谢物。然而，虽然Nafion被认为可以抵抗污垢，但Nafion的浸涂和电沉积都与大量污垢有关，与暴露于脑组织后在裸电极处观察到的水平相似。这些涂层的直接比较确定了每一个独特的电分析性能，可用于指导选择量身定制的目标和环境的具体研究。
Voltammetry is widely used to investigate neurotransmission and other biological processes but is limited by poor chemical selectivity and fouling of commonly used carbon fiber microelectrodes (CFMs). We performed direct comparisons of three key coating materials purported to impart selectivity and fouling resistance to electrodes: Nafion, base-hydrolyzed cellulose acetate (BCA), and fibronectin. We systematically evaluated the impact on a range of electrode parameters. Fouling due to exposure to brain tissue was investigated using an approach that minimizes the use of animals while enabling evaluation of statistically significant populations of electrodes. We find that BCA is relatively fouling resistant. Moreover, detection at BCA-coated CFMs can be tuned by altering hydrolysis times to minimize the impact on sensitivity losses while maintaining fouling resistance. Fibronectin coating is associated with moderate losses in sensitivity after coating and fouling. Nafion imparts increased sensitivity for dopamine and norepinephrine but not serotonin, as well as the anticipated selectivity for cationic neurotransmitters over anionic metabolites. However, while Nafion has been suggested to resist fouling, both dip-coating and electro-deposition of Nafion are associated with substantial fouling, similar to levels observed at bare electrodes after exposure to brain tissue. Direct comparisons of these coatings identified unique electroanalytical properties of each that can be used to guide selection tailored to the goals and environment of specific studies.
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DOI：
10.1124/jpet.102.047134
发表时间：
2003-06-01
影响因子：
3.5
作者：
Ansah, TA;Ramamoorthy, S;Blakely, RD
通讯作者：
Blakely, RD
影响因子：
3
作者：
Borland, LM;Shi, GY;Michael, AC
通讯作者：
Michael, AC
影响因子：
48
作者：
Clark, Jeremy J.;Sandberg, Stefan G.;Wanat, Matthew J.;Gan, Jerylin O.;Horne, Eric A.;Hart, Andrew S.;Akers, Christina A.;Parker, Jones G.;Willuhn, Ingo;Martinez, Vicente;Evans, Scott B.;Stella, Nephi;Phillips, Paul E. M.
通讯作者：
Phillips, Paul E. M.
影响因子：
7.4
作者：
Ge, Shencheng;Woo, Emily;White, James G.;Haynes, Christy L.
通讯作者：
Haynes, Christy L.
DOI：
10.1523/jneurosci.2740-10.2010
发表时间：
2010-11-10
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Baganz N;Horton R;Martin K;Holmes A;Daws LC
通讯作者：
Daws LC