Effect of non-esterified fatty acids on bovine granulosa cell steroidogenesis and proliferation in vitro
Effect of non-esterified fatty acids on bovine granulosa cell steroidogenesis and proliferation in vitro
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DOI：
10.1016/j.anireprosci.2004.09.006
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发表时间：
2005-06-01
影响因子：
2.2
通讯作者：
de Kruif, A
中科院分区：
文献类型：
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作者：
Vanholder, T;Leroy, JLMR;de Kruif, A
在高产奶牛中，产后第一周的负能量平衡（NEB）可能会影响优势卵泡的生长和类固醇生成。由于非酯化脂肪酸（NEFA）的浓度升高，在NEB和被证明是有毒的几种细胞类型，我们研究了三个主要的NEFA的颗粒细胞增殖和类固醇合成在体外的个人和综合影响。将来自大卵泡的颗粒细胞在存在棕榈酸（C16：0）（PA）、硬脂酸（C18：0）（SA）和/或油酸（C18：1）（OA）的无血清培养基中培养两天。加入150、300或500 μ M的PA和SA抑制细胞增殖（P < 0.05），而OA仅在500 μ M时引起这种作用（P < 0.01）。在联合处理（每种脂肪酸150 μ M）中，细胞数也减少（P < 0.01）。这些对细胞数量的抑制作用部分是由于这些NEFA诱导细胞凋亡。用Annexin V-FITC/propidium iodide染色法观察颗粒细胞的凋亡情况。所有剂量的PA、300和500 μ M的SA以及500 μ M的OA均刺激雌二醇-β P的产生（P < 0.05）。用150 μ M的每种脂肪酸组合处理也刺激每104个细胞的雌二醇-17 β产生（P < 0.05）。我们可以得出结论，PA、SA和较小程度的OA在体外调节颗粒细胞增殖和类固醇生成。这些影响可能与高产奶牛产后卵巢功能障碍的发生有关。(c)2004 Elsevier B. V.保留所有权利。
In high-yielding dairy cows, the negative energy balance (NEB) during the first weeks postpartum may influence dominant follicle growth and steroidogenesis. Since non-esterified fatty acid (NEFA) concentrations are elevated during NEB and are shown to be toxic for several cell types, we investigated the individual and combined effects of the three main NEFA's on granulosa cell proliferation and steroidogenesis in vitro. Granulosa cells from large follicles were cultured for two days in serum free medium in the presence of palmitic (C16:0) (PA), stearic (C18:0) (SA) and/or oleic acid (C18:1) (OA). Addition of 150, 300 or 500 mu M of PA and SA inhibited cell proliferation (P < 0.05) while OA only elicited such an effect at 500 mu M (P < 0.01). In the combination treatment (150 mu M of each fatty acid), cell numbers were also reduced (P < 0.01). These inhibitory effects on cell number are partly due to the induction of apoptosis by these NEFA!s, as was demonstrated by annexin V-FITC/propidium iodide staining of the granulosa cells. Oestradiol-beta P production was stimulated by all doses of PA, by 300 and 500 mu M of SA and by 500 mu M of OA (P < 0.05). Combined treatment with 150 mu M of each fatty acid also stimulated oestradiol-17 beta production per 104 cells (P < 0.05). We can conclude that PA, SA and to a lesser degree OA modulate granulosa cell proliferation and steroidogenesis in vitro. These effects may be involved in the occurrence of ovarian dysfunction during the postpartum period in high-yielding dairy cows. (c) 2004 Elsevier B.V. All rights reserved.