Refractometry of microscopic objects with digital holography.
Refractometry of microscopic objects with digital holography.
复制标题
使用数字全息术对微观物体进行折射测量。
DOI：
10.1364/ao.43.004796
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发表时间：
2004
期刊：
影响因子：
1.9
通讯作者：
Mikael Sebesta
中科院分区：
文献类型：
--
作者：
M. Gustafsson;Mikael Sebesta
数字全息术对于微观物体的折射测量具有一些理想的特性，因为它可以从一组曝光中提供物体在所有焦深中的相位和幅度信息。我们表明，图像的幅度部分可用于观察贝克线如何在不同焦深之间移动，从而确定物体是否具有比其周围介质更高或更低的折射率，即浮雕的符号。还表明，一个单相图像提供了一种独立的技术来确定物体与周围介质之间的起伏符号。
Digital holography has some desirable properties for refractometry of microscopic objects since it gives phase and amplitude information of an object in all depths of focus from one set of exposures. We show that the amplitude part of the image can be used to observe how the Becke lines move between different depths of focus and hence determine whether an object has a higher or a lower index of refraction than its surrounding medium, i.e., the sign of the relief. It is also shown that one single-phase image provides an independent technique to determine the sign of relief between an object and the surrounding medium.