A novel R2R3 MYB transcription factor NtMYBJS1 is a methyl jasmonate-dependent regulator of phenylpropanoid-conjugate biosynthesis in tobacco
A novel R2R3 MYB transcription factor NtMYBJS1 is a methyl jasmonate-dependent regulator of phenylpropanoid-conjugate biosynthesis in tobacco
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DOI：
10.1111/j.1365-313x.2006.02719.x
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发表时间：
2006-05-01
期刊：
影响因子：
7.2
通讯作者：
Matsuoka, K
中科院分区：
文献类型：
--
作者：
Gális, I;Simek, P;Matsuoka, K
烟草（Nicotiana tabacum L.）目标代谢和大规模转录组学分析利用BY-2细胞筛选茉莉酸甲酯（MJ）依赖的植物新基因。在代谢水平，我们描述了特定的积累几个苯丙素-多胺共轭物在MJ处理的BY-2细胞。此外，使用含有来自BY-2细胞的表达序列标签（EST）的16 K cDNA微阵列对MJ处理的细胞进行的全局基因表达分析揭示了828个基因在48 h内被MJ处理上调。使用时程表达数据，我们确定了一种新的MJ诱导的R2 R3 MYB型转录因子（NtMYBJS 1），它与核心苯丙素类基因苯丙氨酸解氨酶（PAL）和4-香豆酸：CoA连接酶（4CL）以接近的时间模式共表达。NtMYBJS 1在烟草BY-2细胞中的过表达引起细胞中特异性苯丙素缀合物的积累。随后对NtMYBJS 1转基因株系的微阵列分析显示，在NtMYBJS 1转基因存在下，包括PAL和4CL在内的有限数量的基因被特异性诱导。这些结果，连同反义表达分析和凝胶迁移率变动分析的结果，强烈表明NtMYBJS 1蛋白在烟草MJ信号转导中起作用，诱导苯丙素类生物合成基因和在胁迫期间苯丙素类-多胺缀合物的积累。
Target metabolic and large-scale transcriptomic analyses of tobacco (Nicotiana tabacum L.) Bright Yellow-2 (BY-2) cells were employed to identify novel gene(s) involved in methyl jasmonate (MJ)-dependent function in plants. At the metabolic level, we describe the specific accumulation of several phenylpropanoid-polyamine conjugates in MJ-treated BY-2 cells. Furthermore, global gene expression analysis of MJ-treated cells using a 16K cDNA microarray containing expressed sequence tags (ESTs) from BY-2 cells revealed 828 genes that were upregulated by MJ treatment within 48 h. Using time-course expression data we identified a novel MJ-inducible R2R3 MYB-type transcription factor (NtMYBJS1) that was co-expressed in a close temporal pattern with the core phenylpropanoid genes phenylalanine ammonia-lyase (PAL) and 4-coumarate:CoA ligase (4CL). Overexpression of NtMYBJS1 in tobacco BY-2 cells caused accumulation of specific phenylpropanoid conjugates in the cells. Subsequent microarray analysis of NtMYBJS1 transgenic lines revealed that a limited number of genes, including PAL and 4CL, were specifically induced in the presence of the NtMYBJS1 transgene. These results, together with results of both antisense expression analysis and of gel mobility shift assays, strongly indicate that the NtMYBJS1 protein functions in tobacco MJ signal transduction, inducing phenylpropanoid biosynthetic genes and the accumulation of phenylpropanoid-polyamine conjugates during stress.