An antimicrobial peptide, magainin 2, induced rapid flip-flop of phospholipids coupled with pore formation and peptide translocation
An antimicrobial peptide, magainin 2, induced rapid flip-flop of phospholipids coupled with pore formation and peptide translocation
复制标题
DOI：
10.1021/bi960016v
复制
发表时间：
1996-09-03
期刊：
影响因子：
2.9
通讯作者：
Miyajima, K
中科院分区：
文献类型：
--
作者：
Matsuzaki, K;Murase, O;Miyajima, K
通过使用荧光脂质，即，阴离子N-（7-硝基苯-2-氧杂-1，3-二氮唑-4-基）二棕榈酰-L-α-磷脂酰乙醇胺（NBD-PE），1-油酰-2-[12-（（7-硝基苯并-2-氧杂-1，3-二氮唑-4-基）-氨基）十二烷酰基]-L-α-磷脂酸（C-12-NBD-PA），1-油酰基-2-[12-（（7-硝基苯-2-氧杂-1，3-二唑-4-基）-氨基）十二烷酰基]-L-α-磷脂酰-L-丝氨酸（C-12-NBD-PS）和两性离子1-棕榈酰基-2-[6-（（7-硝基苯并-2-氧杂-1，3-二唑-4-基）氨基）己酰基]-L-α-磷脂酰胆碱（C-6-NBD-PC）。它们在30摄氏度下不存在肽的固有触发半衰期为1.1小时，约1.5小时。大约7小时。8天和>2天。这种肽将荧光脂质的半衰期加快到几分钟。此外，翻转与膜透化和肽易位偶联[Matsuzaki，K.，Murase，O.，Fujii，N.，& Miyajima，K.（1995）Biochemistry，34，6521-6526]，表明孔介导的翻转。翻转率与初始标签条件（外瓣叶标签或内瓣叶标签）无关。根据这些结果，提出了一个模型，其中脂质通过沿着由肽和脂质组成的孔壁的侧向扩散而跨膜移位。一个简单的理论计算可以解释耦合的触发器与透化。
The effect of an antimicrobial peptide, magainin 2, on the flip-flop rates of phospholipids was investigated by use of fluorescent lipids, i.e., anionic N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)dipalmitoyl-L-alpha-phosphatidylethanolamine (NBD-PE), 1-oleoyl-2-[12-((7-nitrobenz-2-oxa-1,3-diazol-4-yl)-amino)dodecanoyl]-L-alpha-phosphatidic acid (C-12-NBD-PA), 1-oleoyl-2-[12-((7-nitrobenz-2-oxa-1,3-diazole-4-yl)-amino)dodecanoyl]-L-alpha-phosphatidyl-L-serine (C-12-NBD-PS), and zwitterionic 1-palmitoyl-2-[6-((7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)caproyl]-L-alpha-phosphatidylcholine (C-6-NBD-PC). Their intrinsic flip-flop half-lives at 30 degrees C in the absence of the peptide were 1.1 h, ca. 7 h, ca. 8 days, and >2 days, respectively. The peptide accelerated the flip-flop half-lives of the fluorescent lipids to an order of minutes. Furthermore, the flip-flop was coupled with the membrane permeabilization and the peptide translocation [Matsuzaki, K., Murase, O., Fujii, N., & Miyajima, K. (1995) Biochemistry, 34, 6521-6526], suggesting pore-mediated flip-flop. The flip-flop rate was independent of the initial labeling conditions (outer leaflet label or inner leaflet label). From these results, a model was proposed, in which the lipids translocate across the membrane by lateral diffusion along the wall of the pores composed of the peptides and the lipids. A simple theoretical calculation could explain the coupling of the flip-flop with the permeabilization.