Structure of N-glycans on the S3- and S6- stylar self-incompatibility ribonucleases of Nicotiana alata
Structure of N-glycans on the S3- and S6- stylar self-incompatibility ribonucleases of Nicotiana alata
复制标题
烟草S3-和S6-花柱自交不亲和性核糖核酸酶上的N-聚糖结构
DOI：
10.1093/glycob/6.6.611
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发表时间：
1996
期刊：
影响因子：
4.3
通讯作者：
A. Bacic
中科院分区：
文献类型：
--
作者：
D. Oxley;S. Munro;D. Craik;A. Bacic
自交不亲和性是许多植物为了防止近亲繁殖而产生的一种机制。茄科植物花柱自交不亲和（S）位点的产物是一系列具有核糖核酸酶活性的糖蛋白。本文报道了烟草（Nicotiana阿拉塔）花柱自交不亲和S-3-和S-6-核糖核酸酶的N-聚糖酶解产物，并通过高pH阴离子交换HPLC进行了分离。共鉴定了14种N-聚糖，并通过电喷雾电离质谱、H-1-NMR光谱、化学降解和甲基化分析进行了表征。这种N-糖基化模式比以前在N-糖基化中发现的模式复杂得多。阿拉塔S-1-和S-2-RNA酶，其中每种仅含有四个N-聚糖。
Self-incompatibility is a mechanism develloped by many plants to prevent inbreeding. The products of the self-incompatibility (S)-locus in the styles of solanaceous plants are a series of glycoproteins with ribonuclease activity, In this study, we report on the N-glycans from the stylar self-incompatibility S-3- and S-6-ribonucleases of Nicotiana alata, which were enzymically released and fractionated by high-pH anion-exchange HPLC. A total of 14 N-glycans were identified and characterized by a combination of electrospray-ionization mass-spectrometry, H-1-NMR spectroscopy, chemical degradation, and methylation analyses. This pattern of N-glycosylation is much more complex than that previously found on the N. alata S-1- and S-2-RNases, each of which contained only four N-glycans.