Detection of Echinococcus multilocularis in domestic dogs of Shiqu County in the summer herding
Detection of Echinococcus multilocularis in domestic dogs of Shiqu County in the summer herding
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DOI：
10.1007/s00436-018-5862-2
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发表时间：
2018-06-01
影响因子：
2
通讯作者：
Nie, Chunying
中科院分区：
文献类型：
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作者：
Hao, Lili;Yang, Aiguo;Nie, Chunying
泡状包虫病（AE）是亚洲最严重的寄生虫病之一。石渠县是我国AE最重要的流行区。本研究的主要目的是了解石渠县夏季放牧期间家犬多房棘球绦虫感染的危险因素。 2016 年 10 月，从 60 名牧场主收集了总共 120 份粪便样本。采用巢式 PCR (nPCR) 扩增多房艾美耳球虫线粒体 12S rRNA 基因区域。结果显示，七五村、一牛村、长沙干马村、德荣马村、蒙易村、下扎村犬只AE感染率分别为5%、5%、10%、20%、10%、5%。需要强调的是，被感染的狗会通过粪便排出虫卵，并可能有捕食寄生虫中间宿主啮齿类动物的习惯，从而再次感染。本次调查证实了石渠地区犬只存在多房阿米巴感染，并揭示了夏季放牧期间感染的危险因素。
Alveolar echinococcosis (AE) is one of the most serious parasitic zoonosis in Asia. Shiqu County is the most important endemic area of AE in China. Our primary objective is to find out the risk factors for Echinococcus multilocularis infection in domestic dogs in Shiqu County during the summer herding period. A total of 120 fecal samples were collected from 60 ranchers in October 2016. Nested PCR (nPCR) was performed to amplify regions of the mitochondrial12S rRNA gene of E. multilocularis. The results showed that the infection rates of AE in dogs from Qiwu, Yiniu, Changshaganma, Derongma, Mengyi, and Xiazha villages were 5, 5, 10, 20, 10, and 5%, respectively. It should be stressed that the infected dogs will shed eggs through feces and may have a habit of preying on rodents, the intermediate host of the parasite, and become re-infected. This investigation confirmed the presence of E. multilocularis infection in dogs in Shiqu and revealed the risk factors associated with the infection during summer herding.