Light-regulated gene expression during maize leaf development.
Light-regulated gene expression during maize leaf development.
复制标题
玉米叶片发育过程中的光基因表达。
DOI：
10.1083/jcb.98.2.558
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发表时间：
1984-02
影响因子：
7.8
通讯作者：
Taylor, W C
中科院分区：
文献类型：
--
作者：
Nelson, T;Harpster, M H;Mayfield, S P;Taylor, W C
我们已经建立了玉米叶片发育期间在光和黑暗中的信使RNA（mRNA）和核酮糖1，5-二磷酸羧化酶（RuBPCase）亚基、磷酸烯醇丙酮酸羧化酶（PEPCase）和捕光叶绿素a/b结合蛋白（LHCP）的多肽的表达时间表。通过与克隆的探针杂交来测量mRNA的水平，并且通过在蛋白质凝胶印迹上的免疫检测来测量蛋白质。所有四种mRNA在叶片中的初始合成遵循不依赖于光的时间表;照明仅影响每个mRNA积累的水平。RuBPCase小亚基和大亚基以及PEPCase多肽的合成也遵循不依赖于光的时间表，该时间表通过光进行定量修改。然而，LHCP多肽的积累绝对需要光照。每种蛋白质的积累密切跟随其mRNA在光照下生长期间的积累。在黑暗生长期间，PEPCase和RuBPCase蛋白与mRNA的比率较高。
We have established schedules of expression during maize leaf development in light and darkness for the messenger RNAs (mRNAs) and polypeptides for ribulose 1,5-bisphosphate carboxylase (RuBPCase) subunits, phosphoenolpyruvate carboxylase (PEPCase), and the light- harvesting chlorophyll a/b-binding protein (LHCP). Levels of mRNAs were measured by hybridization with cloned probes, and proteins were measured by immunodetection on protein gel blots. The initial synthesis in leaves of all four mRNAs follows a light-independent schedule; illumination influences only the level to which each mRNA accumulates. The synthesis of RuBPCase small and large subunits and of PEPCase polypeptides also follows a light-independent schedule which is modified quantitatively by light. However, the accumulation of LHCP polypeptides absolutely requires illumination. The accumulation of each protein closely follows the accumulation of its mRNA during growth in light. Higher ratios of PEPCase and RuBPCase protein to mRNA occur during dark growth.