SELECTION OF A SKIN-TEST ANTIGEN FOR AMERICAN VISCERAL LEISHMANIASIS
SELECTION OF A SKIN-TEST ANTIGEN FOR AMERICAN VISCERAL LEISHMANIASIS
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DOI：
10.4269/ajtmh.1986.35.79
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发表时间：
1986-01-01
影响因子：
3.3
通讯作者：
JONES, TC
中科院分区：
文献类型：
--
作者：
REED, SG;BADARO, R;JONES, TC
研究的目的是检查皮肤试验反应的利什曼抗原在美国内脏利什曼病（AVL）在巴西。我们发现从AVL恢复后，患者对利什曼原虫有阳性迟发型超敏反应。将从杜氏利什曼原虫查加斯前鞭毛体获得的不同量的可溶性提取物与整个利什曼原虫进行比较。D.查加斯前鞭毛体与过去AVL的人。测试的最有效的可溶性制剂含有25和50 μ g利什曼醛蛋白。这些在95%-100%的既往AVL患者中产生了积极反应。在进一步的研究中使用25 μ g蛋白质剂量。该制剂在正常对照、结核病患者或血吸虫病患者中均未产生阳性反应，而在恰加斯病患者中阳性反应< 5%。用等量的L. mexicana amazonensis在过去AVL的人中产生82%的阳性皮肤试验反应。当生活在AVL流行地区的人用25 μ g L. D.查加斯提取物，34.1%产生了积极的测试，在幼儿和48%的成人阳性反应人数较少。只有3.1%的研究人群有AVL病史。我们发现，对可溶性利什曼原虫提取物的阳性迟发型超敏反应是既往AVL感染的敏感和特异性指标。
Studies were designed to examine skin test responses to leishmanial antigens in American visceral leishmaniasis (AVL) in Brazil. We found that after recovery from AVL, patients had positive delayed hypersensitivity reactions to Leishmania. Different amounts of a soluble extract obtained from Leishmania donovani chagasi promastigotes were compared with whole L. d. chagasi promastigotes in persons with past AVL. The most effective soluble preparations tested contained 25 and 50 .mu.g leishmanial protein. These produced positive responses in 95%-100% of the individuals with past AVL. The 25 .mu.g protein dose was used in further studies. This preparation produced no positive responses in either normal controls, tuberculosis patients, or schistosomiasis patients, and < 5% positive responses in persons with Chagas'' disease. The same amount of soluble extract prepared from L. mexicana amazonensis producted 82% positive skin test responses in persons with past AVL. When persons living in an area endemic for AVL were skin tested with the 25 .mu.g preparation of L. d. chagasi extract, 34.1% yielded positive tests with a low number of positive responses in young children and 48% positive in adults. Only 3.1% of the population studied had a history of AVL. We have found that positive delayed hypersensitivity response to a soluble Leishmania extract is a sensitive and specific indicator of previous infection with AVL.