MAMMALIAN ACHAETE-SCUTE HOMOLOG-1 IS TRANSIENTLY EXPRESSED BY SPATIALLY RESTRICTED SUBSETS OF EARLY NEUROEPITHELIAL AND NEURAL CREST CELLS
MAMMALIAN ACHAETE-SCUTE HOMOLOG-1 IS TRANSIENTLY EXPRESSED BY SPATIALLY RESTRICTED SUBSETS OF EARLY NEUROEPITHELIAL AND NEURAL CREST CELLS
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DOI：
10.1101/gad.5.9.1524
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发表时间：
1991-09-01
影响因子：
10.5
通讯作者：
ANDERSON, DJ
中科院分区：
文献类型：
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作者：
LO, LC;JOHNSON, JE;ANDERSON, DJ
使用单克隆抗体，我们已经研究了MASH 1，一个基本的螺旋-环-螺旋蛋白，是一种哺乳动物同源的果蝇achaete-scute蛋白的表达模式。 在果蝇中，无毛鳞片基因是决定神经元子集所必需的。 在大鼠胚胎中，MASH 1表达仅限于神经前体细胞亚群。 MASH 1的诱导先于明显的神经元分化，但在明显的神经元分化后消失。 MASH 1在前脑中通过神经上皮的空间受限结构域表达，在外周神经系统中仅通过交感神经元和肠神经元的前体表达。 早期和瞬时表达的功能，在空间限制的神经前体细胞亚群，观察到的无毛鳞片。 因此，MASH 1和无毛鳞片之间的氨基酸序列保守性反映在表达的细胞类型特异性的平行保守性中，类似于哺乳动物MyoD和果蝇鹦鹉螺的情况。 这些数据支持的想法，螺旋-环-螺旋蛋白可能代表一个进化上保守的细胞类型决定基因家族，其中MASH 1是第一个在脊椎动物中确定的神经特异性成员。
Using monoclonal antibodies, we have examined the expression pattern of MASH1, a basic helix-loop-helix protein that is a mammalian homolog of the Drosophila achaete-scute proteins. In Drosophila, achaete-scute genes are required for the determination of a subset of neurons. In the rat embryo, MASH1 expression is confined to subpopulations of neural precursor cells. The induction of MASH1 precedes, but is extinguished upon, overt neuronal differentiation. MASH1 is expressed in the forebrain by spatially restricted domains of neuroepithelium and in the peripheral nervous system exclusively by precursors of sympathetic and enteric neurons. The features of early and transient expression, in spatially restricted subpopulations of neural precursors, are similar to those observed for achaete-scute. Thus, the amino acid sequence conservation between MASH1 and achaete-scute is reflected in a parallel conservation of cell type specificity of expression, similar to the case of mammalian MyoD and Drosophila nautilus. These data support the idea that helix-loop-helix proteins may represent an evolutionarily conserved family of cell-type determination genes, of which MASH1 is the first neural-specific member identified in vertebrates.