Salivary microRNA: discovery, characterization, and clinical utility for oral cancer detection.
Salivary microRNA: discovery, characterization, and clinical utility for oral cancer detection.
复制标题
DOI：
10.1158/1078-0432.ccr-09-0736
复制
发表时间：
2009-09-01
期刊：
影响因子：
--
通讯作者：
Wong DT
中科院分区：
文献类型：
--
作者：
Park NJ;Zhou H;Elashoff D;Henson BS;Kastratovic DA;Abemayor E;Wong DT
我们之前已经表明，在唾液中发现了转录组，这些mRNA的子组可以用作口腔癌生物标志物。在这项研究中，我们测量了唾液中微小RNA（miRNAs）的存在，并确定了它们作为一组额外的口腔癌生物标志物的潜力。在12名健康对照中，使用逆转录酶-预扩增-定量PCR（RT-preamp-qPCR）测量了总共314个miRNA。在室温下测量内源性和外源性唾液miRNA随时间的降解模式。最后，在50名口腔鳞状细胞癌（OSCC）患者和50名健康对照受试者的唾液中验证了选定的miRNA。我们在整个唾液和上清液中检测到大约50种miRNA。与外源性miRNA相比，内源性唾液miRNA的降解要慢得多。两种miRNAs，miR-125 a和miR-200 a，在OSCC患者唾液中的水平显著低于对照样本（p<0.05）。健康对照的整个唾液和上清液都含有数十种miRNAs，并且与唾液mRNA相似，这些miRNAs似乎也是稳定的。唾液miRNAs可用于口腔癌检测。
We have previously shown that a transcriptome is found in saliva and sub-panels of these mRNAs can be used as oral cancer biomarkers. In this study, we measured the presence of micro-RNAs (miRNAs) in saliva and determined their potential as an additional set of oral cancer biomarkers. A total of 314 miRNAs were measured using reverse transcriptase-preamplification-quantitative PCR (RT-preamp-qPCR) in 12 healthy controls. Degradation pattern of endogenous and exogenous saliva miRNAs were measured at room temperature over time. Finally, selected miRNAs were validated in saliva of 50 oral squamous cell carcinoma (OSCC) patients and 50 healthy matched control subjects. We detected approximately 50 miRNAs in both the whole and supernatant saliva. Endogenous saliva miRNA degraded much slower compared to exogenous miRNA. Two miRNAs, miR-125a and miR-200a, were present in significantly lower levels (p<0.05) in the saliva of OSCC patients than in control samples. Both whole and supernatant saliva of healthy controls contained dozens of miRNAs, and similar to saliva mRNAs, these miRNAs also appear to be stable. Saliva miRNAs can be used for oral cancer detection.