Spinal Cord Transection in the Larval Zebrafish
Spinal Cord Transection in the Larval Zebrafish
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DOI：
10.3791/51479
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发表时间：
2014-05-01
影响因子：
1.2
通讯作者：
Dorsky, Richard I.
中科院分区：
文献类型：
--
作者：
Briona, Lisa K.;Dorsky, Richard I.
由于损伤水平以下缺乏轴突再生以及无法重新启动脊髓神经发生，哺乳动物在脊髓损伤后感觉和运动恢复失败。然而，包括斑马鱼在内的一些羊膜动物即使在脊髓完全横断后也表现出感觉和功能恢复。成年斑马鱼是研究脊髓损伤后再生的既定模型生物，在损伤后 6 周即可恢复感觉和运动。为了利用透明幼虫斑马鱼再生过程的体内分析以及成年斑马鱼无法获得的遗传工具，我们使用斑马鱼幼虫来研究脊髓横断后的再生。在这里，我们展示了一种可重复且可验证地横切幼虫脊髓的方法。横断后，我们的数据显示感觉在受伤后 2 天 (dpi) 开始恢复，3 dpi 时可检测到 C 形弯曲运动，5 dpi 时恢复自由游泳。因此，我们建议幼虫斑马鱼作为成年斑马鱼的伴侣工具，用于研究脊髓损伤后的恢复。
Mammals fail in sensory and motor recovery following spinal cord injury due to lack of axonal regrowth below the level of injury as well as an inability to reinitiate spinal neurogenesis. However, some anamniotes including the zebrafish Danio rerio exhibit both sensory and functional recovery even after complete transection of the spinal cord. The adult zebrafish is an established model organism for studying regeneration following spinal cord injury, with sensory and motor recovery by 6 weeks post-injury. To take advantage of in vivo analysis of the regenerative process available in the transparent larval zebrafish as well as genetic tools not accessible in the adult, we use the larval zebrafish to study regeneration after spinal cord transection. Here we demonstrate a method for reproducibly and verifiably transecting the larval spinal cord. After transection, our data shows sensory recovery beginning at 2 days post-injury (dpi), with the C-bend movement detectable by 3 dpi and resumption of free swimming by 5 dpi. Thus we propose the larval zebrafish as a companion tool to the adult zebrafish for the study of recovery after spinal cord injury.