The S-pombe cytokinesis NDR kinase Sid2 activates Fin1 NIMA kinase to control mitotic commitment through Pom1/Wee1
The S-pombe cytokinesis NDR kinase Sid2 activates Fin1 NIMA kinase to control mitotic commitment through Pom1/Wee1
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DOI：
10.1038/ncb2514
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发表时间：
2012-07-01
影响因子：
21.3
通讯作者：
Hagan, Iain M.
中科院分区：
文献类型：
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作者：
Grallert, Agnes;Connolly, Yvonne;Hagan, Iain M.
有丝分裂退出整合了由有丝分裂激酶启动的磷酸化事件的逆转与将细胞一分为二的受控胞质分裂事件。芽殖酵母的有丝分裂出口网络（MEN）调节这两个过程，而分裂酵母等效物隔膜起始网络（SIN）仅控制胞质分裂的执行。SIN和MEN的组分和结构是高度保守的（1）。目前，它被认为是核心SIN-MEN组件的功能仅限于他们的有丝分裂结束时的特征作用。我们现在表明，NDR（核Dbf 2相关）激酶组成部分的裂变酵母SIN，Sid 2-Mob 1，独立于其他已知的SIN组件在细胞周期的G2期控制有丝分裂承诺的时间。Sid 2-Mob 1通过直接激活NIMA（Never In Mitosis）相关激酶Fin 1促进有丝分裂定型。Fin 1的激活促进了自身的破坏，从而使Fin 1的激活成为G2期的短暂特征。这种Fin 1激活的尖峰调节Pom 1/Cdr 1/Cdr 2几何网络对Wee 1的活性。
Mitotic exit integrates the reversal of the phosphorylation events initiated by mitotic kinases with a controlled cytokinesis event that cleaves the cell in two. The mitotic exit network (MEN) of budding yeast regulates both processes, whereas the fission yeast equivalent, the septum initiation network (SIN), controls only the execution of cytokinesis. The components and architecture of the SIN and MEN are highly conserved(1). At present, it is assumed that the functions of the core SIN-MEN components are restricted to their characterized roles at the end of mitosis. We now show that the NDR (nuclear Dbf2-related) kinase component of the fission yeast SIN, Sid2-Mob1, acts independently of the other known SIN components in G2 phase of the cell cycle to control the timing of mitotic commitment. Sid2-Mob1 promotes mitotic commitment by directly activating the NIMA (Never In Mitosis)-related kinase Fin1. Fin1's activation promotes its own destruction, thereby making Fin1 activation a transient feature of G2 phase. This spike of Fin1 activation modulates the activity of the Pom1/Cdr1/Cdr2 geometry network towards Wee1.