Molecular characterization of Streptococcus pneumoniae serotype 12F isolates associated with rural community outbreaks in Alaska.
Molecular characterization of Streptococcus pneumoniae serotype 12F isolates associated with rural community outbreaks in Alaska.
复制标题
与阿拉斯加农村社区疫情相关的肺炎链球菌血清型 12F 分离株的分子特征。
DOI：
10.1128/jcm.02880-12
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发表时间：
2013
影响因子：
9.4
通讯作者：
Hennessy,ThomasW
中科院分区：
文献类型：
--
作者：
Zulz,Tammy;Wenger,JayD;Rudolph,Karen;Robinson,DAshley;Rakov,AlexeyV;Bruden,Dana;Singleton,RosalynJ;Bruce,MichaelG;Hennessy,ThomasW
2003 - 2006年和2006 - 2008年在阿拉斯加两个邻近农村地区分别发现12F型肺炎链球菌引起的侵袭性肺炎球菌病（IPD）暴发。对1986年至2009年IPD监测数据以及1998年至2004年和2008年至2009年的携带调查数据进行了审查，以确定血清型12F的传播模式。对所有可用的分离株进行脉冲场凝胶电泳，并通过附加的遗传分型方法对选定的分离株进行表征。血清型12F IPD在1986年至2008年期间在阿拉斯加发生了两波。虽然安克雷奇几乎每年都有病例发生，但在农村地区，12F - IPD的发病率比安克雷奇高10至20倍，疾病高峰之间往往间隔多年，通常由单一显性基因克隆引起。携带主要发生在成年人中，但在农村暴发的早期，大多数携带发生在18岁以下的人群中。在农村地区，12F的携带在疫情暴发后完全消失。不同的12F克隆体似乎已被偶然地引入农村人口，在免疫能力为naïve的年轻人口中广泛传播（导致持续1至3年的IPD暴发），然后迅速从人口中消失。较大的人口中心可能是这些克隆的储存库。这种流行病学模式与一种高毒力但具有免疫原性的肺炎球菌相一致。
Outbreaks of invasive pneumococcal disease (IPD) caused by Streptococcus pneumoniae serotype 12F were observed in two neighboring regions of rural Alaska in 2003 to 2006 and 2006 to 2008. IPD surveillance data from 1986 to 2009 and carriage survey data from 1998 to 2004 and 2008 to 2009 were reviewed to identify patterns of serotype 12F transmission. Pulsed-field gel electrophoresis was performed on all available isolates, and selected isolates were characterized by additional genetic subtyping methods. Serotype 12F IPD occurred in two waves in Alaska between 1986 and 2008. While cases of disease occurred nearly every year in Anchorage, in rural regions, 12F IPD occurred with rates 10- to 20-fold higher than those in Anchorage, often with many years between disease peaks and generally caused by a single predominant genetic clone. Carriage occurred predominantly in adults, except early in the rural outbreaks, when most carriage was in persons <18 years old. In rural regions, carriage of 12F disappeared completely after outbreaks. Different 12F clones appear to have been introduced episodically into rural populations, spread widely in young, immunologically naïve populations (leading to outbreaks of IPD lasting 1 to 3 years), and then disappeared rapidly from the population. Larger population centers might have been the reservoir for these clones. This epidemiologic pattern is consistent with a highly virulent, but immunogenic, form of pneumococcus.