Asymmetric reduction of 2-octanone in water/organic solvent biphasic system with Baker's yeast FD-12
Asymmetric reduction of 2-octanone in water/organic solvent biphasic system with Baker's yeast FD-12
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DOI：
10.1016/j.enzmictec.2006.10.016
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发表时间：
2007-04-03
影响因子：
3.4
通讯作者：
Guo, Yang-Hao
中科院分区：
文献类型：
--
作者：
Li, Yong-Ning;Shi, Xian-Ai;Guo, Yang-Hao
在水介质中，2-辛酮与面包酵母FD-12的不对称还原反应在较高浓度时受到底物和产物的严重抑制，导致产率和e.e.值较低。目标产品(S)-2-辛醇的价值。本工作对七种水/有机溶剂两相体系进行了测定。在所考察的两相体系中，FD-12在水/正十二烷体系中表现出最好的耐受性、催化活性和对映体选择性。FD-12在正十二烷体系中的代谢活性保留率为98%，活性保留率为91.6%。考察了体积比V-AQ/V-org、温度、pH值和底物浓度对2-辛醇产率和e.E.(S)值的影响。在最佳条件下(水/正十二烷两相体系，V-AQ/V-Org 20/5，pH 8.0，32℃，2-辛酮浓度500 mM)下，还原96h后，2-辛醇的最终浓度为105.60 mM，eE(S)为89.38%。采用FD-12的水/正十二烷两相体系有利于生产最终浓度高、e.E.(S)值高的(S)-2-辛醇。(C)2006 Elsevier Inc.保留所有权利。
in the aqueous medium the asymmetric reduction of 2-octanone with Baker's yeast FD-12 was inhibited severely by the substrate and the product at higher concentrations, which resulted in a low yield and a low e.e. value of the object product (S)-2-octanol. Seven water/organic solvent biphasic systems were assayed in this work. FD-12 showed the best tolerance, catalytic activity and enantioselectivity in the water/n-dodecane system among the biphasic systems assayed. The metabolic activity retention (MAR) and viability of FD-12 in the n-dodecane containing system were 98% and 91.6%, respectively. The influence of the volume ratio V-aq/V-org, temperature, pH value and the substrate concentration on the yield of 2-octanol and the e.e.(S) value was investigated. Under the optimum conditions (in the water/n-dodecane biphasic system, V-aq/V-org 20/5, pH 8.0, 32 degrees C, 2-octanone concentration 500 mM), the final concentration of 2-octanol and the e.e.(S) value reached 105.60 MM and 89.38%, respectively, after 96 h reduction. The water/n-dodecane biphasic system with FD- 12 was advantageous for the production of (S)-2-octanol of high final concentration and high e.e.(S) value. (c) 2006 Elsevier Inc. All rights reserved.