CaGnT-III, an N-acetylglucosaminyl- transferase gene from pepper, positively regulates plants tolerance to abiotic stresses by enhancing antioxidant ability
CaGnT-III, an N-acetylglucosaminyl- transferase gene from pepper, positively regulates plants tolerance to abiotic stresses by enhancing antioxidant ability
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CaGnT-III 是一种来自辣椒的 N-乙酰氨基葡萄糖转移酶基因，通过增强抗氧化能力积极调节植物对非生物胁迫的耐受性
DOI：
10.1016/j.envexpbot.2021.104394
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发表时间：
2021
影响因子：
5.7
通讯作者：
Lu Minghui
中科院分区：
文献类型：
--
作者：
Liu Haibo;Chen Tao;Zhu Zuting;Zhao Fang;Liang Minmin;Lu Minghui
As a common post-translational modification pathway, glycosylation plays important roles in maintaining proteins’ inherent conformations and normal functions. The contribution of coreN-glycans formed in endoplasmic reticulum (ER) to plant tolerance against salt stress has been well-studied; however, the knowledge about the involvement of complexN-glycans synthesized in Golgi apparatus is limited. Here, we report that a pepperN-acetylglucosaminyl-transferase III geneCaGnT-III, which takes part in the maturation of complexN-glycans, can respond to heat, salt, and drought stress. Furthermore, silencing ofCaGnT-IIIcompromises pepper tolerance and its overexpression enhances Arabidopsis tolerance, to the above abiotic stresses. Under stressed conditions, compared to those in the control with empty vector, inCaGnT-IIIsilenced pepper seedlings, the increase of MDA (malondialdehyde) accumulation and the decrease of total chlorophyll content were aggravated, while the enhancement of proline content, CAT (catalase) and APX (ascorbate peroxidase) activities, and stress-related genes expression were weakened. On the contrary, overexpression ofCaGnT-IIIin Arabidopsis presented the opposite results. Therefore, we speculate thatCaGnT-IIIgene positively regulates the formation of plants tolerance to abiotic stress by enhancing antioxidant ability.
