Characterization of HaRNAV, a single-stranded RNA virus causing lysis of Heterosigma akashiwo (Raphidophyceae)
Characterization of HaRNAV, a single-stranded RNA virus causing lysis of Heterosigma akashiwo (Raphidophyceae)
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DOI：
10.1046/j.1529-8817.2003.01162.x
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发表时间：
2003-04-01
影响因子：
2.9
通讯作者：
Suttle, CA
中科院分区：
文献类型：
--
作者：
Tai, V;Lawrence, JE;Suttle, CA
HaRNAV是一种感染赤潮异弯藻Heterosigma akashiwo（Hada）Hada ex Hada et Chihara的新型病毒，根据形态学、病理学、核酸类型、结构蛋白和感染的宿主菌株范围对其进行了表征。HaRNAV是一种25-nm单链RNA（ssRNA）病毒，基因组大小约为9100个核苷酸。这是第一次报告的ssRNA病毒，导致溶解的浮游植物物种。病毒颗粒对氯仿敏感，并含有至少五种结构蛋白，表观大小为24至34 kDa。HaRNAV感染引起内质网肿胀，并且子代病毒颗粒在宿主的细胞质中组装，通常呈晶体阵列。用15株H.明石窝与日本沃茨、东北太平洋和西北大西洋隔绝。HaRNAV引起来自东北太平洋的三个菌株和来自日本的两个菌株的裂解，但没有来自西北大西洋的菌株。HaRNAV的特征表明HaRNAV是一种新型的浮游植物病毒，但与属于Equiviridae的植物病毒和其他已知的ssRNA病毒有一些相似之处。然而，进一步的基因组分析是必要的，以确定任何系统发育关系。HaRNAV的发现进一步说明了H. Akashiwo病毒病原体，更重要的是藻类-病毒病原体以及环境中病毒-宿主相互作用的复杂性。
HaRNAV, a novel virus that infects the toxic bloom-forming alga Heterosigma akashiwo (Hada) Hada ex Hada et Chihara, was characterized based on morphology, pathology, nucleic acid type, structural proteins, and the range of host strains that it infects. HaRNAV is a 25-nm single-stranded RNA (ssRNA) virus with a genome size of approximately 9100 nucleotides. This is the first report of an ssRNA virus that causes lysis of a phytoplankton species. The virus particle is sensitive to chloroform and contains at least five structural proteins ranging in apparent size from 24 to 34 kDa. HaRNAV infection causes swelling of the endoplasmic reticulum and progeny virus particles assemble in the cytoplasm of the host, frequently in crystalline arrays. The infectivity of HaRNAV was tested against 15 strains of H. akashiwo isolated from Japanese waters, the Northeast Pacific, and the Northwest Atlantic. HaRNAV caused lysis of three strains from the Northeast Pacific and two strains from Japan but none from the Northwest Atlantic. The characterization of HaRNAV demonstrates that HaRNAV is a novel type of phytoplankton virus but has some similarities with plant viruses belonging to the Sequiviridae and to other known ssRNA viruses. Further genomic analysis, however, is necessary to determine any phylogenetic relationships. The discovery of HaRNAV emphasizes the diversity of H. akashiwo viral pathogens and, more importantly, algal-virus pathogens and the complexity of virus-host interactions in the environment.