CABYR is essential for fibrous sheath integrity and progressive motility in mouse spermatozoa
CABYR is essential for fibrous sheath integrity and progressive motility in mouse spermatozoa
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DOI：
10.1242/jcs.193151
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发表时间：
2016-12-01
影响因子：
4
通讯作者：
Ikawa, Masahito
中科院分区：
文献类型：
--
作者：
Young, Samantha A. M.;Miyata, Haruhiko;Ikawa, Masahito
钙结合酪氨酸磷酸化调节蛋白（CABYR）在几项体外研究中与精子的生理功能有关。它也被认为是男性弱精子症的潜在病因和诊断工具。已知CABYR定位于鞭毛主片中的附属结构纤维鞘。利用CRISPR-Cas9技术，我们已经敲除了小鼠中的这个基因，以了解它在男性生育力中的作用。Cabr基因敲除的雄性小鼠表现出严重的生育力低下，精子活力缺陷，以及纤维鞘的显着解体。此外，在Cabr基因敲除精子中观察到异常的双微管结构，表明纤维鞘对轴丝的正确组织是重要的。我们的研究结果表明，这是CABYR的作用，在形成的纤维鞘，是必不可少的男性生育力。
Ca2+-binding tyrosine-phosphorylation-regulated protein (CABYR) has been implicated in sperm physiological function in several in vitro studies. It has also been implicated as a potential cause of and diagnostic tool in asthenozoospermic human males. CABYR is known to be localized to the fibrous sheath, an accessory structure in the flagellar principal piece. Utilizing the CRISPR-Cas9 technology, we have knocked out this gene inmice to understand its role inmale fertility. Cabyr-knockout male mice showed severe subfertility with a defect in sperm motility as well as a significant disorganization in the fibrous sheath. Further, abnormal configuration of doublet microtubules was observed in the Cabyr-knockout spermatozoa, suggesting that the fibrous sheath is important for the correct organization of the axoneme. Our results show that it is the role of CABYR in the formation of the fibrous sheath that is essential for male fertility.