Utilization of digital differential display to identify differentially expressed genes related to rumen development
Utilization of digital differential display to identify differentially expressed genes related to rumen development
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DOI：
10.1111/asj.12448
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发表时间：
2016-04-01
影响因子：
2
通讯作者：
Roh, Sang-Gun
中科院分区：
文献类型：
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作者：
Kato, Daichi;Suzuki, Yutaka;Roh, Sang-Gun
本研究旨在通过计算机数字差异显示技术筛选与瘤胃上皮细胞发育相关的候选基因。利用NCBI的UniGene数据库中的DDD技术，对牛瘤胃、网胃、重瓣、皱胃等组织中基于EST的基因表达谱进行了分析。从组织文库中筛选出110个在瘤胃中高表达的候选基因。对9头日本黑公犊牛（5周龄断奶前犊牛：n=3; 15周龄断奶犊牛：n=6）的瘤胃、网胃、重瓣和皱胃中所有候选基因中的11个基因的表达水平进行了分析。在这11个基因中，只有3-羟基-3-甲基戊二酰辅酶A合成酶2（HMGCS 2），醛酮还原酶家族1，成员C1样（AKR 1C 1），和脂肪酸结合蛋白3（FABP 3）的基因表达水平在犊牛断奶前和断奶后的瘤胃中表现出显着的变化。这些结果表明，计算机DDD分析可用于筛选与瘤胃发育相关的候选基因，并且这3个基因在瘤胃中表达水平的变化可能是由断奶、衰老或两者共同引起的。(c)2015年日本动物科学学会
This study aimed to identify the genes associated with the development of the rumen epithelium by screening for candidate genes by digital differential display (DDD) in silico. Using DDD in NCBI's UniGene database, expressed sequence tag (EST)-based gene expression profiles were analyzed in rumen, reticulum, omasum, abomasum and other tissues in cattle. One hundred and ten candidate genes with high expression in the rumen were derived from a library of all tissues. The expression levels of 11 genes in all candidate genes were analyzed in the rumen, reticulum, omasum and abomasum of nine Japanese Black male calves (5-week-old pre-weaning: n=3; 15-week-old weaned calves: n=6). Among the 11 genes, only 3-hydroxy-3-methylglutaryl-CoA synthase 2 (HMGCS2), aldo-keto reductase family 1, member C1-like (AKR1C1), and fatty acid binding protein 3 (FABP3) showed significant changes in the levels of gene expression in the rumen between the pre- and post-weaning of calves. These results indicate that DDD analysis in silico can be useful for screening candidate genes related to rumen development, and that the changes in expression levels of three genes in the rumen may have been caused by weaning, aging or both. (c) 2015 Japanese Society of Animal Science