Increased Expression and Aberrant Localization of Mucin 13 in Metastatic Colon Cancer
Increased Expression and Aberrant Localization of Mucin 13 in Metastatic Colon Cancer
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DOI：
10.1369/0022155412460678
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发表时间：
2012-11-01
影响因子：
3.2
通讯作者：
Chauhan, Subhash C.
中科院分区：
文献类型：
--
作者：
Gupta, Brij K.;Maher, Diane M.;Chauhan, Subhash C.
MUC13是一种新发现的跨膜粘蛋白。虽然已知MUC13在卵巢癌和胃癌中过表达，但关于MUC13在转移性结肠癌中的表达的信息有限。本文采用免疫组化（IHC）方法，利用一种新型抗MUC13单克隆抗体（MAb，克隆ppz0020）研究MUC13在结肠癌中的表达谱。本研究使用了一组结肠癌样本和组织芯片，其中包含邻近的正常、非转移性结肠癌、转移性结肠癌和肝转移组织，以研究MUC13的表达模式。免疫组化分析显示，MUC13在非转移性结肠癌样本中的表达显著高于（p < 0.001），而在邻近正常组织中的表达微弱或极低。有趣的是，与非转移性结肠癌和邻近正常结肠样本相比，转移性结肠癌和肝转移组织样本的细胞质和细胞核MUC13表达显著（p < 0.05）升高。此外，细胞质和细胞核MUC13的表达与较大和低分化的肿瘤相关。六种结肠癌细胞系中有四种也在RNA和蛋白质水平上表达MUC13。这些研究表明，MUC13在转移性结肠癌中的表达显著增加，并提示MUC13异常定位（细胞质和核表达）与转移性结肠癌之间存在相关性。（中国生物医学工程学报，2012）
MUC13 is a newly identified transmembrane mucin. Although MUC13 is known to be overexpressed in ovarian and gastric cancers, limited information is available regarding the expression of MUC13 in metastatic colon cancer. Herein, we investigated the expression profile of MUC13 in colon cancer using a novel anti-MUC13 monoclonal antibody (MAb, clone ppz0020) by immunohistochemical (IHC) analysis. A cohort of colon cancer samples and tissue microarrays containing adjacent normal, non-metastatic colon cancer, metastatic colon cancer, and liver metastasis tissues was used in this study to investigate the expression pattern of MUC13. IHC analysis revealed significantly higher (p < 0.001) MUC13 expression in non-metastatic colon cancer samples compared with faint or very low expression in adjacent normal tissues. Interestingly, metastatic colon cancer and liver metastasis tissue samples demonstrated significantly (p < 0.05) higher cytoplasmic and nuclear MUC13 expression compared with non-metastatic colon cancer and adjacent normal colon samples. Moreover, cytoplasmic and nuclear MUC13 expression correlated with larger and poorly differentiated tumors. Four of six tested colon cancer cell lines also expressed MUC13 at RNA and protein levels. These studies demonstrate a significant increase in MUC13 expression in metastatic colon cancer and suggest a correlation between aberrant MUC13 localization (cytoplasmic and nuclear expression) and metastatic colon cancer. (J Histochem Cytochem 60:822-831, 2012)