Gamma-tubulin in Chlamydomonas: characterization of the gene and localization of the gene product in cells.
Gamma-tubulin in Chlamydomonas: characterization of the gene and localization of the gene product in cells.
复制标题
衣藻中的γ-微管蛋白：基因的表征和基因产物在细胞中的定位。
DOI：
10.1002/(sici)1097-0169(1999)42:4
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Palevitz,BA
中科院分区：
文献类型：
--
作者：
Silflow,CD;Liu,B;LaVoie,M;Richardson,EA;Palevitz,BA
In addition to their role in nucleating the assembly of axonemal microtubules, basal bodies often are associated with a microtubule organizing center (MTOC) for cytoplasmic microtubules. In an effort to define molecular components of the basal body apparatus inChlamydomonas reinhardtii, genomic and cDNA clones encoding γ‐tubulin were isolated and sequenced. The gene, present in a single copy in theChlamydomonasgenome, encodes a protein with a predicted molecular mass of 52,161 D and 73% and 65% conservation with γ‐tubulin from higher plants and humans, respectively. To examine the distribution of γ‐tubulin in cells, a polyclonal antibody was raised against two peptides contained within the protein. Immunoblots ofChlamydomonasproteins show a major cross‐reaction with a protein of Mr 53,000. InChlamydomonascells, the antibody stains the basal body apparatus as two or four spots at the base of the flagella and proximal to the microtubule rootlets. During cell division, two groups of fluorescent dots separate and localize to opposite ends of the mitotic apparatus. They then migrate during cleavage to positions known to be occupied by basal bodies. Changes in γ‐tubulin localization during the cell cycle are consistent with a role for this protein in the nucleation of microtubules of both the interphase cytoplasmic array and the mitotic spindle. Immunogold labeling of cell sections showed that γ‐tubulin is closely associated with the basal bodies. The flagellar transition region was also labeled, possibly indicating a role for γ‐tubulin in assembly of the central pair microtubules of the axoneme. Cell Motil. Cytoskeleton 42:285–297, 1999. © 1999 Wiley‐Liss, Inc.
