Construction of a high-density linkage map and fine mapping of QTLs for growth and gonad related traits in blunt snout bream.
Construction of a high-density linkage map and fine mapping of QTLs for growth and gonad related traits in blunt snout bream.
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钝口鳊生长及性腺相关性状高密度连锁图谱构建及QTL精细定位
DOI：
10.1038/srep46509
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发表时间：
2017-04-19
影响因子：
4.6
通讯作者：
Gao ZX
中科院分区：
文献类型：
--
作者：
Wan SM;Liu H;Zhao BW;Nie CH;Wang WM;Gao ZX
基于SNPs的高密度遗传图谱对于精细定位控制鱼类特定性状的基因座至关重要。利用限制性酶切位点相关DNA标签测序（RAD-Seq）技术，我们在团头鲂基因组中发现了42，784个均匀分布的SNPs。基于2个亲本和187个种内杂交后代，将14，648个高置信度SNP分配到24个母系和父系连锁群（LGs）中。该图谱总长度为3258.38cM，5676个有效位点间的平均距离为0.57cM，是首次报道的M的高密度遗传图谱。钝头虫。QTL分析共检测到8个阳性数量性状位点。其中，5个QTL解释了≥35%的生长性状表型变异，3个QTL解释了≥16%的性腺相关性状表型变异。共有176个标记在斑马鱼基因组中有显著的命中，几乎所有的24条假定染色体的M。弱视头与斑马鱼染色体处于相对保守的同线状态。几乎所有的M。除第4号染色体定位于斑马鱼的两条染色体上外，其它染色体均为1：1对应，这是由于两种鱼类染色体数目的差异造成的。
High-density genetic maps based on SNPs are essential for fine mapping loci controlling specific traits for fish species. Using restriction-site associated DNA tag sequencing (RAD-Seq) technology, we identified 42,784 SNPs evenly distributed across theMegalobrama amblycephalagenome. Based on 2 parents and 187 intra-specific hybridization progenies, a total of 14,648 high-confidence SNPs were assigned to 24 consensus linkage groups (LGs) of maternal and paternal map. The total length of the integrated map was 3,258.38 cM with an average distance of 0.57 cM among 5676 effective loci, thereby representing the first high-density genetic map reported forM. amblycephala. A total of eight positive quantitative trait loci (QTLs) were detected in QTL analysis. Of that, five QTL explained ≥35% of phenotypic variation for growth traits and three QTL explained ≥16% phenotypic variation for gonad related traits. A total of 176 mapped markers had significant hits in the zebrafish genome and almost all of the 24 putative-chromosomes ofM. amblycephalawere in relatively conserved synteny with chromosomes of zebrafish. Almost allM. amblycephalaand zebrafish chromosomes had a 1:1 correspondence except for putative-chromosome 4, which mapped to two chromosomes of zebrafish caused by the difference in chromosome numbers between two species.