A genomic approach to understanding Heliothis and Helicoverpa resistance to chemical and biological insecticides
A genomic approach to understanding Heliothis and Helicoverpa resistance to chemical and biological insecticides
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DOI：
10.1098/rstb.1998.0323
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发表时间：
1998-10-29
期刊：
影响因子：
--
通讯作者：
Trowell, S
中科院分区：
文献类型：
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作者：
Heckel, DG;Gahan, LJ;Trowell, S
基因组学是对整个基因组的结构和功能进行比较研究。尽管对任何害虫的基因组进行完整测序是遥远的未来，但基因组学方法在研究杀虫剂抗药性的机制方面可能是有用的。我们描述了这两个世界上最具破坏性的害虫飞蛾属(鳞翅目)的这种策略。全基因组连锁作图提供了主要和次要抗性基因的位置。定位克隆发现了新的抗性基因，即使机制还不太清楚，比如对苏云金芽孢杆菌毒素的抗性。锚定基因座为比较相关物种间的基因组和抗性机制的遗传结构提供了参考点。总的来说，这些工具能够描述相关但独立的基因组对环境中常见的杀虫剂选择压力的进化反应。它们还提供了对特定抗性基因的靶向管理有用的信息，甚至可能加速在鳞翅目中寻找新的杀虫靶标家族。
Genomics is the comparative study of the structure and function of entire genomes. Although the complete sequencing of the genome of any insect pest is far in the future, a genomic approach can be useful in the study of mechanisms of insecticide resistance. We describe this strategy for Heliothis and Helicoverpa, two of the most destructive genera of pest moths (Lepidoptera) worldwide. Genome-wide linkage mapping provides the location of major and minor resistance genes. Positional cloning identifies novel resistance genes, even when the mechanisms are poorly understood, as with resistance to Bacillus thuringiensis toxins. Anchor loci provide the reference points for comparing the genomes and the genetic architecture of resistance mechanisms among related species. Collectively, these tools enable the description of the evolutionary response of related, but independent, genomes to the common selective pressure of insecticides in the environment. They also provide information that is useful for targeted management of specific resistance genes, and may even speed the search for families of novel insecticidal targets in Lepidoptera.