Immunohistochemical evidence for coexistence of glycine and GABA in nerve terminals on cat spinal motoneurones: an ultrastructural study.
Immunohistochemical evidence for coexistence of glycine and GABA in nerve terminals on cat spinal motoneurones: an ultrastructural study.
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猫脊髓运动神经元神经末梢中甘氨酸和 GABA 共存的免疫组织化学证据：一项超微结构研究。
DOI：
10.1097/00001756-199404000-00009
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发表时间：
1994
期刊：
影响因子：
1.7
通讯作者：
Staffan Cullheim
中科院分区：
文献类型：
--
作者：
Göran Örnung;Oleg Shupliakov;O. Ottersen;Jon Storm;Staffan Cullheim
先前的电生理学和药理学研究表明甘氨酸和γ -氨基丁酸（GABA）作为递质参与反射通路介导猫脊髓运动神经元的复发性抑制。利用包埋后免疫金技术和抗戊二醛偶联氨基酸抗体，发现猫脊髓运动核中甘氨酸和gaba免疫反应性神经末梢与α运动神经元大小的细胞体接触，以及周围的神经细胞。这些终端中有很大一部分同时含有甘氨酸-和GABA-LI。这些末端突触囊泡上免疫标记的富集强烈表明这两种氨基酸都被用作传递物质。
Previous electrophysiological and pharmacological studies have suggested the involvement of both glycine and gamma-aminobutyric acid (GABA) as transmitters in the reflex pathway mediating recurrent inhibition of cat spinal motoneurones. By use of the postembedding immunogold technique and antibodies against glutaraldehyde-conjugated amino acids it is shown that glycine- and GABA-immunoreactive nerve terminals occur in contact with cell bodies of alpha motoneurone size, as well as in the surrounding neuropil in the motor nuclei of the cat spinal cord. A substantial proportion of these terminals harbours both glycine- and GABA-LI. The enrichment of immunolabelling over synaptic vesicles in such terminals strongly suggests that both of these amino acids are used as transmitter substances.