Transmission electron microscopy studies on pig gastric mucin and its interactions with chitosan
Transmission electron microscopy studies on pig gastric mucin and its interactions with chitosan
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DOI：
10.1016/0144-8617(95)00105-0
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发表时间：
1995-11-01
影响因子：
11.2
通讯作者：
Davis, SS
中科院分区：
文献类型：
--
作者：
Fiebrig, I;Harding, SE;Davis, SS
透射电子显微镜已被用于显示猪胃粘蛋白的高度纯化制剂的分子结构和组织（摩尔质量M类似于9 x10（6）g/mol，通过低速沉降平衡测定），通过两种完全独立的方法制备用于显微镜检查。通过在云母上空气干燥（在50%甘油的存在下）以及在丙酮/CO2中的临界点干燥来制备用于成像的样品。数据似乎与粘蛋白的公认线性模型一致，并且与沿着多肽主链的可变糖基化程度沿着区域一致，具有松散或无规卷曲的整体构象。然后探索了这种粘蛋白在具有潜在的粘膜粘附聚合物壳聚糖（M类似于160，000 g/mol）的稀溶液混合物中的行为，并且证明了清楚的相互作用，这与在分析超离心中使用沉降速度分析的稀溶液测量一致。
Transmission electron microscopy has been employed to visualize the molecular structure and organization of a highly purified preparation of pig gastric mucin (molar mass M similar to 9x10(6) g/mol, as determined by low speed sedimentation equilibrium), prepared for microscopy by two completely independent methods. Samples were prepared for imaging by air drying on mica (in the presence of 50% glycerol) as well as critical point drying in acetone/CO2. The data appear consistent with the accepted linear model for mucins, and are consistent with regions of variable degrees of glycosylation along the polypeptide backbone chain, with the overall conformation that of a loose or random coil. The behaviour of this mucin in a dilute solution mixture with the potential mucoadhesive polymer chitosan (M similar to 160,000 g/mol) was then explored, and a clear interaction was demonstrated, consistent with dilute solution measurements using sedimentation velocity analysis in the analytical ultracentrifuge.