Skeletal analysis and characterization of gene expression related to pattern formation in developing limbs of Japanese silkie fowl
Skeletal analysis and characterization of gene expression related to pattern formation in developing limbs of Japanese silkie fowl
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DOI：
10.2141/jpsa.43.126
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发表时间：
2006-04-01
影响因子：
1.5
通讯作者：
Ono, Tamao
中科院分区：
文献类型：
--
作者：
Arisawa, Kenjiro;Yazawa, Shigenobu;Ono, Tamao
日本乌骨鸡（Ukokkei）是一种具有多种独特特征的家禽品种。骨骼分析显示，日本乌骨鸡胚胎在第 32 至 36 阶段的翼芽中明显短暂地出现了一个额外的软骨手指。相比之下，腿部的一个额外的手指在骨骼元素方面与数字 2 相似。整体原位杂交分析表明，Shh、Bmp-2 和 Hoxd-13 基因在第 26 阶段的腿芽额外指的假定区域中表达。在日本乌骨鸡胚胎发育中的翼芽中，Shh 和 Hoxd-13 的表达在前部区域（包括额外指的假定区域）也很明显。因此，日本乌鸡值得进一步研究，作为研究肢体发育的潜在模型动物。
The Japanese Silkie (Ukokkei) is a breed of domestic fowl with several unique characteristics. Skeletal analysis revealed that an extra cartilaginous digit was apparent transiently in the wing bud of Japanese Silkie embryos between stages 32 and 36. In contrast, an extra digit of the leg resembled digit 2 in terms of its skeletal elements. Whole-mount in situ hybridization analysis revealed that Shh, Bmp-2, and Hoxd-13 genes are expressed in the presumptive region of the extra digit in the leg buds at stage 26. Expression of Shh and Hoxd-13 was also apparent in the anterior region, including the presumptive area of the extra digit, in the developing wing bud of Japanese Silkie embryos. The Japanese Silkie fowl thus warrants further investigation as a potential model animal for the study of limb development.