A sensitive fluorescent nanosensor for chloramphenicol based on molecularly imprinted polymer-capped CdTe quantum dots
A sensitive fluorescent nanosensor for chloramphenicol based on molecularly imprinted polymer-capped CdTe quantum dots
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DOI：
10.1002/bio.3003
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发表时间：
2016-05-01
期刊：
影响因子：
2.9
通讯作者：
Manzoori, Jamshid L.
中科院分区：
文献类型：
--
作者：
Amjadi, Mohammad;Jalili, Roghayeh;Manzoori, Jamshid L.
利用分子印迹二氧化硅纳米球嵌入CdTe量子点（CdTe@SiO2@MIP）构建了一种新型荧光纳米传感器，用于氯霉素（CAP）的检测和定量。采用反相微乳液法在亲水性CdTe量子点上合成分子印迹聚合物（MIP），制备了印迹传感器。通过荧光光谱、紫外-可见吸收光谱、红外光谱和透射电镜对所得CdTe@SiO2@MIP纳米粒子进行了表征。它们保留了母体量子点的48%的荧光量子产率。CAP对CdTe@SiO2@MIP的荧光有显著的猝灭作用，可能是通过电子转移机制实现的。在最佳条件下，CdTe@SiO2@MIP的相对荧光强度在40-500 μ g L-1浓度范围内随CAP的增加而降低，符合Stern-Volmer型方程。相应的检测限为5.0 μ g L-1。该方法精密度的日内和日间值均为
A novel fluorescent nanosensor using molecularly imprinted silica nanospheres embedded CdTe quantum dots (CdTe@SiO2@MIP) was developed for detection and quantification of chloramphenicol (CAP). The imprinted sensor was prepared by synthesis of molecularly imprinting polymer (MIP) on the hydrophilic CdTe quantum dots via reverse microemulsion method using small amounts of solvents. The resulting CdTe@SiO2@MIP nanoparticles were characterized by fluorescence, UV-vis absorption and FT-IR spectroscopy and transmission electron microscopy. They preserved 48% of fluorescence quantum yield of the parent quantum dots. CAP remarkably quenched the fluorescence of prepared CdTe@SiO2@MIP, probably via electron transfer mechanism. Under the optimal conditions, the relative fluorescence intensity of CdTe@SiO2@MIP decreased with increasing CAP by a Stern-Volmer type equation in the concentration range of 40-500 mu g L-1. The corresponding detection limit was 5.0 mu g L-1. The intra-day and inter-day values for the precision of the proposed method were all