Three-dimensional observation of connective tissue of bovine Masseter muscle under concentrate -: and roughage-fed conditions by using immunohistochemical/confocal laser-scanning microscopic methods
Three-dimensional observation of connective tissue of bovine Masseter muscle under concentrate -: and roughage-fed conditions by using immunohistochemical/confocal laser-scanning microscopic methods
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DOI：
10.1111/j.1750-3841.2007.00413.x
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发表时间：
2007-08-01
影响因子：
3.9
通讯作者：
Gotoh, T.
中科院分区：
文献类型：
--
作者：
Nakamura, Y.-N.;Iwamoto, H.;Gotoh, T.
我们研究了精饲料组和粗饲料组（分别为 C 组和 R 组）的日本黑色小母牛（n = 6）咬肌 (MA) 结缔组织成分的变化。 C组实验小母牛屠宰时的体重(272.3+/-22.3kg)高于R组(213.8+/-27.5kg)(P<0.05)。然而，C组和R组之间MAmuscle的肌肉重量和肌纤维直径（浅层和深层）没有差异。相反，R组的总咀嚼时间比C组长（P < 0.05），C组和R组的MAmuscle仅由I型肌纤维组成。使用免疫组织化学/共聚焦、激光扫描显微镜，主要在肌束膜中观察到I型胶原蛋白，在肌束膜中观察到V型和VI型胶原蛋白。和两组的肌内膜。两组均仅在肌内膜中观察到 IV 型胶原和层粘连蛋白。然而，R组的肌束膜和肌内膜中III型胶原和纤连蛋白非常明显。观察到C组和R组的肌束膜中的结缔组织成分形成板状层。另一方面，在肌内膜周围的肌纤维中看到蜂窝状结缔组织成分。特别是，在R组的肌束膜和肌内膜中强烈观察到纤连蛋白。这些结果表明MA肌肉中的结缔组织成分响应咀嚼而存在不同的发育变化。免疫组织化学/共焦激光扫描显微方法可用于研究骨骼肌中结缔组织成分之间的结构关系。
We investigated changes in connective tissue components of masseter (MA) muscle in Japanese black heifers (n= 6) in concentrate- and roughage-fed groups (groups C and R, respectively). Body weight, at slaughter of experimental heifers in group C (272.3 +/- 22.3kg) was higher (P < 0.05) than that of group R(213.8 +/- 27.5 kg). However, muscle weight and myofiber diameter (superficial and deep layers) of MAmuscle did not differ between groups C and R. In contrast, total mastication duration of group R was longer (P < 0.05) than that of group C, MAmuscle of groups C and R was composed only of type I myofiber. Using immunohistochemical/confocal, laser-scanning microscopy , type I collagen was observed mainly in perimysium, and type V and VI collagen were observed in perimysium. and endomysium of both groups. Type IV collagen and laminin were observed only In the endomysium in both groups. However, type ill collagen and fibronectin were strongly apparent in the perimysium and endomysium in group R. Connective tissue components in the perimysium of groups C and R were observed to form plate-shaped layers. On the other hand, honeycomb-shaped connective tissue components were seen in the endomysium-surrounded muscle fibers. In particular, fibronectin was strongly observed in the perimysium and endomysium in group R. These results indicate that there are different developmental changes among connective tissue components in MA muscle in response to mastication. The immunohistochemical/confocal laser-scanning microscopic method Is useful to, investigate the structural relationship among connective tissue components In skeletal muscle.