Synthesis and evaluation of a novel fluorescent chemosensor for glutathione based on a rhodamine B and N-[4-(carbonyl) phenyl]maleimide conjugate and its application in living cell imaging
Synthesis and evaluation of a novel fluorescent chemosensor for glutathione based on a rhodamine B and N-[4-(carbonyl) phenyl]maleimide conjugate and its application in living cell imaging
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DOI：
10.1016/j.dyepig.2016.08.063
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Xiaofeng Bao
中科院分区：
文献类型：
--
作者：
Hai Shu;Xiaolei Wu;Baojing Zhou;Yingbin Han;Mingjie Jin;Jing Zhu;Xiaofeng Bao
A novel rhodamine B spirolactam derivative bearing an N-[4-(carbonyl)phenyl] maleimide moiety (L1) was designed, synthesized and structurally characterized to develop a chemosensor. The interactions of L1 with amino acids and metal ions were studied by UV–vis absorption and fluorescence spectroscopy. L1 exhibited a highly sensitive and selective turn-on fluorescence response toward glutathione (GSH) over other biological species in EtOH/HEPES (3:2, v/v, 0.1 mM, pH 7.34) solution. The detection limit of GSH by L1 was 0.219 μM. Intracellular imaging applications demonstrated that L1 can be used as a fluorescent probe for the detection of GSH in HepG-2 and HUVEC cells.
