Determination of sophoridine and related lupin alkaloids using tris(2,2 '-bipyridine)ruthenium electrogenerated chemiluminescence
Determination of sophoridine and related lupin alkaloids using tris(2,2 '-bipyridine)ruthenium electrogenerated chemiluminescence
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DOI：
10.1016/s0003-2670(02)00546-9
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发表时间：
2002-08
影响因子：
6.2
通讯作者：
Xi Chen;C. Yi;Mei-Jin Li;Xin Lu;Zhen Li;Peiwei Li;Xiao-ru Wang
中科院分区：
文献类型：
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作者：
Xi Chen;C. Yi;Mei-Jin Li;Xin Lu;Zhen Li;Peiwei Li;Xiao-ru Wang
研究了三（2,2′-联吡啶）钌（II） （Ru(bpy)32+）和槐定碱（SRI）、苦参碱（MT）、槐醇（SR）和槐果碱（SC）在pH为9.0的碱性缓冲溶液中的电化学发光。发光主要是由于生物碱化合物上的叔氨基与Ru(bpy)32+在配有22.1mm2的玻碳圆盘电极的薄层流动电池中在+1.50V（相对于Ag/AgCl）电位下发生电氧化反应引起的。610nm的发光波长证实了ECL是由Ru(bpy)32+ *对其基态产生的。它们的ECL强度受取代基特征、氢在β-碳原子上的三维构象等因素的影响。从计算数据中得到的电离势进一步证实了实验结果。此外，还研究了影响四种生物碱测定和HPLC分离的因素。
Electrogenerated chemiluminescences (ECLs) based on tris(2,2′-bipyridine)ruthenium(II) (Ru(bpy)32+) and lupin alkaloids, for instance, sophoridine (SRI), matrine (MT), sophoranol (SR) and sophocarpine (SC) in an aqueous alkaline buffer solution (pH 9.0) are studied. The light emission is mainly caused by an electro-oxidation reaction between tertiary amino group on the alkaloid compounds and Ru(bpy)32+in a thin layer flow cell equipped with a glassy carbon disc electrode (22.1mm2) at the potential of +1.50V (versus Ag/AgCl). The luminescence wavelength of 610nm confirmed that ECL is caused by Ru(bpy)32+∗to its ground state. ECL intensities of these lupin alkaloids are affected by the substituent character, three-dimensional conformation of hydrogen on β-carbon atom. Ionization potentials taken from calculation data further confirm the experimental results. In addition, the factors affecting the determination and HPLC separation of the four alkaloids are also investigated.