The use of bacterial membrane fractions for the detection of Campylobacter species in shellfish
The use of bacterial membrane fractions for the detection of Campylobacter species in shellfish
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DOI：
10.1111/j.1745-4581.1997.tb00165.x
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发表时间：
1997-07-01
影响因子：
--
通讯作者：
Wekell, MM
中科院分区：
文献类型：
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作者：
Abeyta, C;Trost, PA;Wekell, MM
选择的富集肉汤补充有酶Oxyrase（R），一种来源于大肠杆菌的膜结合酶。大肠杆菌的回收率进行了评估，弯曲杆菌在正常大气条件下从贝类。结果表明，氧化酶可用于贝类中弯曲杆菌的回收。氧化酶对弯曲杆菌生长的影响取决于培养基体积和表体积面积等物理参数。例如，在比较Stomacher（R）400密封袋和250 mL和500 mL螺旋盖锥形瓶中各种培养基体积中弯曲杆菌属的生长（低至高水平，1 × 10（1 - 6）/mL）时，使用密封袋未回收弯曲杆菌。然而，在锥形瓶中回收了所有水平的弯曲杆菌。氧化酶对C.来自太平洋牡蛎（Crossostrea gigas）的空肠。在一项研究中，太平洋牡蛎被允许摄取1 × 10（6）个C细胞。每毫升空肠在人工海水水族箱系统中持续5 - 7小时。取出牡蛎并在4 ℃下储存24天。牡蛎的摄取范围为0.4至114个细胞/克。每隔一段时间，牡蛎被取出并分析C。空肠通过使用Oxyrase方法和FDA/细菌学分析手册标准方法。这些方法的比较表明，Oxyrase方法与FDA官方方法一样可靠。氧化酶也可用于回收市售牡蛎壳中天然存在的弯曲杆菌。为了测量方法的重现性，在委内瑞拉中部大学（加拉加斯）进行了内部初步合作研究。参与者由来自中美洲和南美洲不同国家的6组微生物学家组成。所有组均成功使用Oxyrase回收空肠弯曲菌和结肠弯曲菌。我们的研究结果表明，氧化酶为贝类弯曲杆菌的生长提供了微好氧条件。
Selected enrichment broths supplemented with the enzyme Oxyrase(R) a membrane-bound enzyme derived from E. coli were evaluated for recoveries of Campylobacter under normal atmospheric conditions from shellfish. Results indicate that Oxyrase is useful for the recovery of Campylobacter from shellfish. Effect of Oxyrase on growth of Campylobacter were dependent upon physical parameters such as media volume and surface volume area. For example, in the comparision of growth of Campylobacter sp (low to high Levels, 1 x 10(1 to 6)/mL) in various media volumes in Stomacher(R)400 closure bags and 250 mL and 500 mL screw-capped Erlenmeyer flasks, no campylobacters were recovered using the closure bags. However, all levels of Campylobacter were recovered in the Erlenmeyer flasks. Oxyrase was useful for the recovery of C. jejuni from Pacific oysters (Crossostrea gigas). In one study, Pacific shellstock oysters were allowed to take up 1 x 10(6) cells of C. jejuni per ml for a period of 5 to 7 h in an artificial seawater aquarium system. Oysters were removed and stored at 4C for 24 days. Uptake in oysters ranged from 0.4 to 114 cells/g. At intervals, oysters were removed and analyzed for C. jejuni by using the Oxyrase method and the FDA/Bacteriological Analytical Manual standard method. Comparison of these methods showed that the Oxyrase method was as reliable as the official FDA method. Oxyrase was also useful for recovering naturally occurring Campylobacter in market oyster shellstock. To measure reproducibility of the method, an in-house preliminary collaborative study was conducted in the University of Central Venezuela (Caracas). Participants consisted of 6 groups of three microbiologist each from various countries in Central and South America. All groups were successful in recovering Campylobacter jejuni and Campylobacter coli using Oxyrase. Our results indicate that Oxyrase provides microaerobic conditions for growth of Campylobacter from shellfish.