Porogen-induced surface modification of nano-fibrous poly(L-lactic acid) scaffolds for tissue engineering
Porogen-induced surface modification of nano-fibrous poly(L-lactic acid) scaffolds for tissue engineering
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DOI：
10.1016/j.biomaterials.2006.03.008
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发表时间：
2006-07-01
期刊：
影响因子：
14
通讯作者：
Ma, PX
中科院分区：
文献类型：
--
作者：
Liu, XH;Won, YJ;Ma, PX
除了组织工程支架合适的孔结构之外，支架制造后通常需要进行表面修饰，以增强细胞与合成材料之间的相互作用。在这项研究中，开发了一种新颖的一步法来制造表面改性的纳米纤维聚（（L）-乳酸）（NF-PLLA）支架。首先，通过非表面活性剂乳化、溶剂萃取、冷冻干燥制备表面光滑的明胶球。然后使用明胶球作为致孔剂制备三维 NF-PLLA 支架。在制造过程中，明胶分子被捕获在支架表面。衰减全反射-傅里叶变换红外光谱（ATR-FTIR）光谱和X射线光电子能谱分析证明了聚合物支架表面存在明胶分子。支架表面上明胶的量由明胶溶液的溶剂混合物的组成控制。用明胶球制备的支架的压缩模量比用相同尺寸范围的不规则明胶颗粒制备的支架高3倍以上。经过两周的培养，表面修饰显着改善了初始细胞粘附和增殖。 SEM 图像表明，细胞在细胞接种后 1 天铺展在明胶包埋的支架上，与对照上的球形或纺锤形形态形成鲜明对比。此外，体外培养两周后，在表面修饰的支架上观察到比对照支架上更多的基质分泌。总之，这种方法提供了一种简单的一步法来制造表面改性的胶原蛋白样 NF-PLLA 支架，该支架具有改善的细胞粘附和增殖能力。 (c) 2006 Elsevier Ltd. 保留所有权利。
In addition to suitable pore architecture of a tissue-engineering scaffold, surface modification after scaffoldin fabrication is often needed to enhance the interactions between cells and the synthetic material. In this study, a novel one-step process was developed to fabricate surface-modified nano-fibrous poly((L)-lactic acid) (NF-PLLA) scaffolds. First, gelatin spheres with smooth surface were prepared by non-surfactant emulsification, solvent extraction, and freeze-drying. A three-dimensional NF-PLLA scaffold was then fabricated by using gelatin spheres as porogen. Gelatin molecules were entrapped onto the scaffold surface during the fabrication process. Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) spectroscopy and X-ray photoelectron spectroscopy analysis demonstrated the existence of gelatin molecules on the surface of the polymer scaffold. The amount of gelatin on the scaffold surface was controlled by the composition of the solvent mixture of gelatin solution. The compressive modulus of scaffold prepared with gelatin spheres was more than three times higher than that prepared with irregular gelatin particles of the same size range. The surface modification significantly improved initial cell adhesion and proliferation over a 2-week culture. SEM images indicated that cells spread on the gelatin-en trapped scaffolds in contrast to spherical or spindle morphology on the control 1 day after cell seeding. Furthermore, more matrix secretion was observed on the surface-modified scaffolds than on the control after 2 weeks of in vitro cultivation. In conclusion, this approach provides a simple one-step process to fabricate surface-modified collagen-like NF-PLLA scaffolds, which have improved cell adhesion and proliferation. (c) 2006 Elsevier Ltd. All rights reserved.