The expression and clinical significance of Omi/Htra2 in hepatocellular carcinoma.
The expression and clinical significance of Omi/Htra2 in hepatocellular carcinoma.
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DOI：
10.5754/hge12524
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发表时间：
2012-10
影响因子：
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通讯作者：
Zongquan Xu;Xiao-ping Chen;Cheng Peng;E. Liu;Yunguang Li;Chang-hai Li;J. Niu
中科院分区：
文献类型：
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作者：
Zongquan Xu;Xiao-ping Chen;Cheng Peng;E. Liu;Yunguang Li;Chang-hai Li;J. Niu
目的探讨Omi/HtrA2在肝细胞癌中的表达及其临床意义。方法采用免疫组织化学、RT-PCR和Western印迹方法检测Omi/HtrA2在肝细胞癌、癌旁组织和正常肝组织中的表达。免疫组织化学方法检测肝细胞癌组织中缺氧诱导因子-1α(HIF-1α)的表达。同时，回顾分析Omi/HtrA2的表达与患者生存时间的关系。结果Omi/HtrA2在肝细胞癌中高表达，并与肝细胞癌分化程度、肿瘤大小、门静脉侵犯、临床分期及有无淋巴结转移有关。我们还观察到在肝细胞癌中OMi1/HtrA2的表达与HIF-1α的表达呈显著负相关。Kaplan-Meier估计表明，Omi/HtrA2阳性患者的生存时间比Omi/HtrA2阴性患者长得多。用COX回归模型进行单因素和多因素分析表明，Omi/HtrA2表达是影响预后的重要因素。结论肝癌细胞可能需要Omi/HtrA2表达才能诱导细胞凋亡，Omi/HtrA2可能是判断原发性肝细胞癌预后的重要指标。
BACKGROUND/AIMS To investigate Omi/HtrA2 expression and its clinical significance in hepatocellular carcinoma. METHODOLOGY We analyzed Omi/HtrA2 expression in hepatocellular carcinoma, paracancerous tissues and normal hepatic tissues by immunohistochemistry, RT-PCR and Western blot. Hypoxia-inducible factor-1α (HIF-1α) expression was also detected in hepatocellular carcinoma samples by immunohistochemistry. Meanwhile, we retrospectively analyzed the relationship between Omi/HtrA2 expression and the survival times of the patients. RESULTS We found that Omi/HtrA2 overexpressed in hepatocellular carcinoma and was correlated with hepatocellular carcinoma differentiation, tumor size, portal vein invasion, clinical stage and lymph node metastasis. We also observed a significant inverse correlation between the expression of Omi,/HtrA2 and HIF-1α in hepatocellular carcinoma. The Kaplan-Meier estimates showed that patients who were Omi/HtrA2 positive had much longer survival times than those who were Omi/HtrA2 negative. Both univariate and multivariate analysis using the Cox regression model indicated that Omi/HtrA2 expression was a significant factor for prognosis. CONCLUSIONS Hepatocellular carcinoma cells may need Omi/HtrA2 expression for apoptosis and Omi/HtrA2 might be an important prognostic marker for primary hepatocellular carcinoma.