Silencing of Kv4.1 potassium channels inhibits cell proliferation of tumorigenic human mammary epithelial cells
Silencing of Kv4.1 potassium channels inhibits cell proliferation of tumorigenic human mammary epithelial cells
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DOI：
10.1016/j.bbrc.2009.04.108
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发表时间：
2009-06-26
影响因子：
3.1
通讯作者：
Lee, So Yeong
中科院分区：
文献类型：
--
作者：
Jang, Soo Hwa;Choi, Changsun;Lee, So Yeong
钾离子通道活性已被证明能促进癌细胞的增殖。本研究探讨了Kv4.1通道在不朽和致瘤性人乳腺上皮细胞中的作用。Kv4.1蛋白表达与致瘤性呈正相关。此外，转染靶向Kv4.1 mRNA的sirna可抑制致瘤性乳腺上皮细胞的增殖。利用从人乳腺癌组织中分离的mRNA进行的实验表明，Kv4.1 mRNA的表达水平随肿瘤的分期而变化。与正常组织相比，T2和T3期Kv4.1蛋白表达增加。这些结果表明Kv4.1在致瘤性人乳腺上皮细胞的增殖中起作用。此外，Kv4.1表达升高可能有助于作为乳腺肿瘤分期的诊断标志物，Kv4.1的选择性阻滞剂可能有助于抑制肿瘤细胞的增殖。(C) 2009爱思唯尔公司版权所有。
Potassium channel activity has been shown to facilitate cell proliferation in cancer cells. In the present study, the role of Kv4.1 channels in immortal and tumorigenic human mammary epithelia] cells was investigated. Kv4.1 protein expression was positively correlated with tumorigenicity. Moreover, transfection with siRNAs targeting Kv4.1 mRNA suppressed proliferation of tumorigenic mammary epithelial cells. Experiments using mRNA isolated from human breast cancer tissues revealed that the level of Kv4.1 mRNA expression varied depending on the stage of the tumor. Kv4.1 protein expression increased during stages T2 and T3 compared to normal tissue. These results demonstrated that Kv4.1 plays a role in proliferation of tumorigenic human mammary epithelial cells. In addition, elevated Kv4.1 expression may be useful as a diagnostic marker for staging mammary tumors and selective blockers of Kv4.1 may serve to suppress tumor cell proliferation. (C) 2009 Elsevier Inc. All rights reserved.