Ia-bearing bone marrow-cultured macrophages induce antigen-specific helper T cells for antibody synthesis.
Ia-bearing bone marrow-cultured macrophages induce antigen-specific helper T cells for antibody synthesis.
复制标题
携带 Ia 的骨髓培养巨噬细胞诱导抗原特异性辅助 T 细胞进行抗体合成。
DOI：
10.4049/jimmunol.123.2.612
复制
发表时间：
1979
影响因子：
4.4
通讯作者：
R. Gisler
中科院分区：
文献类型：
--
作者：
A. Stern;P. Erb;R. Gisler
在补充了集落刺激因子 (CSF) 的骨髓细胞 (BMC) 培养物中，辅助细胞会发育，能够诱导特定的辅助 T 细胞。这些辅助细胞在培养 4 天后变得有效，不仅可以在贴壁细胞群中找到，还可以在非贴壁细胞群中找到。另一方面，在没有 CSF 的 BMC 培养物中获得的具有辅助细胞诱导能力的辅助细胞非常少。活性 BMC 衍生细胞类型已被证明携带 Ia 表面抗原，因为用抗 Ia 血清和补体预处理消除了这些细胞在辅助 T 细胞诱导中像巨噬细胞一样发挥功能的能力。此外，这些培养物中功能性辅助细胞的出现与携带 Ia 的细胞的存在相一致。
In bone marrow cell (BMC) cultures supplemented with colony-stimulating factor (CSF), accessory cells develop that are capable of inducing specific helper T cells. These accessory cells become effective after 4 days in culture and can be found not only in the adherent but also in the nonadherent cell population. On the other hand, very few accessory cells with helper cell-inducing capacity are obtained in BMC cultures without CSF. The active BMC-derived cell type has been shown to carry Ia surface antigen, since pretreatment with anti-Ia serum and complement abolished the capacity of these cells to function like macrophages in helper T cell induction. Moreover, the appearance of functional accessory cells in these cultures coincided with the presence of Ia-bearing cells.