Frequent allelic imbalances at 8p and 11q22 in oral and oropharyngeal epithelial dysplastic lesions
Frequent allelic imbalances at 8p and 11q22 in oral and oropharyngeal epithelial dysplastic lesions
复制标题
DOI：
10.1016/j.cancergencyto.2005.01.004
复制
发表时间：
2005-08-01
影响因子：
--
通讯作者：
Wong, DTW
中科院分区：
文献类型：
--
作者：
Zhou, XF;Jordan, RCK;Wong, DTW
等位基因失衡是口腔鳞状细胞癌（SCC）的特征，并导致该疾病的肿瘤发生。我们之前的研究表明，与 q22.3 相似的染色体区域 8p 和 11q22.2 是头颈部 SCC 杂合性丢失 (LOH) 的常见位点。在这里，我们探讨了 27 例口腔上皮发育不良病变中这些区域的等位基因失衡模式。之前报道的头颈发育不良中常见的 LOH (9p21) 也进行了检查。利用激光捕获显微切割（LCM）技术从存档的临床组织中收获同质细胞群，从而大大提高遗传评估的灵敏度、准确性和可靠性。与 q22.3 和 9p21 类似的 8p、11q22.2 上的等位基因不平衡（LOH 和微卫星不稳定性）在一个或多个位点上观察到，分别有 66.7%、63.0% 和 63.0% 的病例。我们的结果表明，与 q22.3 相似的 8p、11q22.2 和 9p21 是口腔癌前不典型增生中常见的等位基因失衡区域，并表明这些区域中存在抑癌基因。 (c) 2005 Elsevier Inc. 保留所有权利。
Allelic imbalance is characteristic of oral squamous cell carcinoma (SCC) and contributes to the tumorigenesis of this disease. Our previous studies suggest that chromosome regions 8p and 11q22.2 similar to q22.3 are frequent sites of loss of heterozygosity (LOH) in head and neck SCC. Here, we explored the allelic imbalance pattern of these regions in 27 cases of oral epithelial dysplastic lesions. A previously reported frequent LOH (9p21) in head and neck dysplasia was also examined. Laser capture microdissection (LCM) technology was utilized to harvest homogenous cell populations from archived clinical tissues and thus greatly enhancing the sensitivity, accuracy and reliability of genetic assessment. The allelic imbalance (LOH and microsatellite instability) on 8p, 11q22.2 similar to q22.3, and 9p21 were observed at one or more loci in 66.7 %, 63.0 %, and 63.0 % of cases, respectively. Our results demonstrate that 8p, 11q22.2 similar to q22.3, and 9p21 are frequent allelic imbalance regions in oral premalignant dysplasia and suggest the presence of tumor suppressor genes in these regions. (c) 2005 Elsevier Inc. All rights reserved.