JAK2, STAT3 and SOCS3 gene expression in women with and without breast cancer
JAK2, STAT3 and SOCS3 gene expression in women with and without breast cancer
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DOI：
10.1016/j.gene.2014.06.025
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发表时间：
2014-08-15
期刊：
影响因子：
3.5
通讯作者：
Gonzalez-Banales, Juan Manuel
中科院分区：
文献类型：
--
作者：
Santillan-Benitez, Jonnathan Guadalupe;Mendieta-Zeron, Hugo;Gonzalez-Banales, Juan Manuel
简介：乳腺癌是一种由构成乳腺的细胞基因组中积累的变化引起的疾病。Janus kinase2(JAK2)/信号转导和转录激活因子3(STAT3)信号通路已被报道参与乳腺癌的发生发展，并被肥胖时升高的脂肪细胞因子如瘦素激活。目的：本研究旨在明确JAK2、STAT3和SOCS3基因表达是否与体重指数(BMI)和乳腺癌有关。应用RT-qPCR方法检测26例乳腺癌和43例乳腺良性病变组织中JAK2、STAT3和SOCS3基因的表达水平。结果：STAT3基因在乳腺癌患者中的表达增加(P<0.05)。
Introduction: Breast cancer is a disease that arises from the accumulation of alterations in the genome of cells that make up the mammary gland. The Janus kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3) signaling pathway has been reported to participate in the development of breast cancer and is activated by adipocytokines such as leptin, which are elevated in obesity. In contrast, alterations in suppressor of cytokine signaling 3 (SOCS3) gene expression have been found in patients with decreased breast cancer metastasis.Objective: The current study sought to identify whether JAK2, STAT3 and SOCS3 gene expression is associated with body mass index (BMI) and breast cancer.Methods: This was a cross-sectional prospective study. JAK2, STAT3 and SOCS3 gene expression levels were determined using RT-qPCR from the biopsies of 26 patients with breast cancer and 43 patients with benign breast lesions. We compared the expression of these genes, relative to the housekeeping genes, ACTB and GAPDH, against BMI, clinical stage and immunohistochemistry.Results: STAT3 gene expression was increased in breast cancer patients (p