RESISTANCE AND ABROGATION OF RESISTANCE TO CUTANEOUS LEISHMANIASIS IN RECONSTITUTED BALB-C NUDE-MICE
RESISTANCE AND ABROGATION OF RESISTANCE TO CUTANEOUS LEISHMANIASIS IN RECONSTITUTED BALB-C NUDE-MICE
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DOI：
10.1038/icb.1981.47
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发表时间：
1981-01-01
期刊：
影响因子：
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通讯作者：
HANDMAN, E
中科院分区：
文献类型：
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作者：
MITCHELL, GF;CURTIS, JM;HANDMAN, E
正常BALB/c小鼠和低胸腺BALB/c裸鼠对巨噬细胞内原生动物寄生虫热带利什曼原虫的分离株感染高度敏感，发展为严重的皮肤疾病。用大量BALB/c淋巴样细胞重建的裸鼠也是易感的，但当注射少量细胞时（例如，5倍。106脾加肠系膜淋巴结细胞）。慢性皮肤病的易感性是完全不同的，在最低限度的重建BALB/c裸鼠比BALB/c小鼠与正常或接近正常的T细胞补体。保留的最小再造裸体容易再感染，但损伤会愈合。最低限度重建的耐药裸鼠已被用作细胞和血清成分的筛选，这些成分可能导致皮肤和血清成分的慢性化，这些成分可能导致感染的BALB/c小鼠皮肤疾病的慢性化。来自慢性感染BALB/c的少量富含T细胞的脾细胞在裸鼠中没有保护作用，并且在混合实验中消除了少量同基因正常小鼠脾细胞的保护作用。用抗Lyt 2试剂处理慢性感染小鼠脾细胞不会影响细胞消除耐药性的能力。一部分最低限度重建的裸鼠在多次注射慢性感染小鼠的血清后易患皮肤病。这种耐药性的消除与慢性感染小鼠细胞介导的耐药性不同，因为它们不一致。T细胞依赖性免疫应答（但可能不是Lyt 2 + Ts细胞依赖性效应）可能导致遗传易感BALB/c小鼠感染的慢性化，并抑制该小鼠品系病变的愈合。虽然数据提出了这种可能性，但在这一系列实验中没有获得令人信服的证据表明TH细胞依赖性阻断抗体或其他血清成分参与了BALB/c小鼠的慢性疾病。
Normal BALB/c mice and hypothymic BALB/c nude mice are highly susceptible to infection with an isolate of the intramacrophage protozoan parasite, Leishmania tropica, developing severe cutaneous disease. Nude mice reconstituted with large numbers of BALB/c lymphoid cells are also susceptible but are resistant when injected with low numbers of cells (e.g., 5 .times. 106 spleen plus mesenteric lymph node cells). Susceptibility to chronic cutaneous disease is quite different in minimally reconstituted BALB/c nude mice than in BALB/c mice with a normal or near-normal complement of T cells. Recovered minimally reconstituted nudes are susceptible to reinfection but the lesions heal. The minimally reconstituted, resistant nude mouse has been used as a screen for cellular and serum components which may contribute to chronicity of cutaneous and serum components which may contribute to chronicity of cutaneous disease in infected BALB/c mice. Low numbers of T cell-enriched spleen cells from chronically infected BALB/c are not protective in nude mice and abrogate the protective effect of low numbers of syngeneic normal mouse spleen cells in a mix experiment. Treatment of chronically infected mouse spleen cells with anti-Lyt2 reagents does not affect the capacity of the cells to abrogate resistance. A proportion of minimally reconstituted nude mice were susceptible to cutaneous disease after multiple injections of serum from chronically infected mice. This abrogation of resistance differed from that mediated by cells of chronically infected mice in being inconsistent. T cell-dependent immune responses (but perhaps not Lyt2+ Ts cell-dependent effects) may contribute to chronicity of infection in genetically susceptible BALB/c mice and inhibit healing of lesions in this mouse strain. Although the possibility is raised by the data, no compelling evidence was obtained in this series of experiments that TH cell-dependent blocking antibodies, or other serum components, are involved in perpetuating chronic disease in BALB/c mice.