A COLORIMETRIC METHOD FOR ESTIMATING SERUM TRIGLYCERIDES
A COLORIMETRIC METHOD FOR ESTIMATING SERUM TRIGLYCERIDES
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DOI：
10.1016/0009-8981(68)90041-7
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发表时间：
1968-01-01
影响因子：
5
通讯作者：
FLETCHER, MJ
中科院分区：
文献类型：
--
作者：
FLETCHER, MJ
本文介绍一种测定血清甘油酯的比色法。血清甘油酯皂化释放的甘油被氧化为甲醛，甲醛与乙酰丙酮反应生成黄色二氢二甲基吡啶衍生物，吸收波长为405 mμ。结果与Zilversmit-Van Handel方法的结果有很好的相关性。正常受试者的值范围为每100 ml 40-155 mg三油酸甘油酯（1.36-5.25 mequiv/l），通过Student检验，男性和女性的平均值之间的差异具有统计学意义。
A colorimetric method for the estimation of serum glycerides is presented. The glycerol released by saponification of the serum glycerides is oxidized to formaldehyde which reacts with acetylacetone to form a yellow dihydrolutidine derivative absorbing at 405 mμ. The results correlate well with those given by the ZilversmitVan Handel method. Values for normal subjects range between 40–155 mg triolein per 100 ml (1.36–5.25 mequiv/1), the difference between the mean values for males and females being statistically significant by Student'sttest.