LOW-DOSE ULTRAVIOLET-B IRRADIATION DEPLETES HUMAN EPIDERMAL LANGERHANS CELLS
LOW-DOSE ULTRAVIOLET-B IRRADIATION DEPLETES HUMAN EPIDERMAL LANGERHANS CELLS
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DOI：
10.1111/j.1365-2133.1993.tb03330.x
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发表时间：
1993-12-01
影响因子：
10.3
通讯作者：
HAWK, JLM
中科院分区：
文献类型：
--
作者：
MURPHY, GM;NORRIS, PG;HAWK, JLM
我们研究了低剂量单色 UVB 照射（295 +/- 5 nm）对人体皮肤中 CD1a 表皮朗格汉斯细胞表面标志物表达的影响，其生物学效果相当于 12 次日光浴床过程中通常发生在皮肤上的照射。在 5 名受试者中，29±5 nm 处的 1.5 个最小红斑剂量 (MED) 使其表达减少了 50%。在另外五名受试者中，单次 1.5 MED 剂量、隔天 10 个等份的 1.5 MED 剂量以及以之前使用的辐照度十分之一的单次 1.5 MED 剂量递送至不同部位，也导致 CD1a 表达发生变化但显着的降低约 30-50%。因此，根据 CD1a 表达的测量，低剂量 UVB 照射，无论是快速还是缓慢接受，都会显着且大致相同地消耗人表皮朗格汉斯细胞数量。
We have examined the effects of low-dose monochromatic UVB irradiation (295 +/- 5 nm), biologically equivalent to that generally incident on the skin during a 12-session sun-bed course, on the expression of the CD1a epidermal Langerhans cell surface marker in human skin in vivo. In five subjects, 1.5 minimal erythema doses (MEDs) at 29 5 nm depleted its expression by 50%. In five further subjects, a single 1.5 MED dose, 1.5 MEDs in 10 equal fractions on alternate days, and a single 1.5 MED dose at one-tenth the previously used irradiance, delivered to separate sites, also led to variable but significant depletion of CD1a expression of around 30-50%. Thus, low-dose UVB irradiation, whether received rapidly or slowly, appears significantly and approximately equally to deplete human epidermal Langerhans cell numbers as measured by CD1a expression.