Mouse D1Pas1, a DEAD-box RNA helicase, is required for the completion of first meiotic prophase in male germ cells
Mouse D1Pas1, a DEAD-box RNA helicase, is required for the completion of first meiotic prophase in male germ cells
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DOI：
10.1016/j.bbrc.2016.07.109
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发表时间：
2016-09-16
影响因子：
3.1
通讯作者：
Ogura, Atsuo
中科院分区：
文献类型：
--
作者：
Inoue, Hiroki;Ogonuki, Narumi;Ogura, Atsuo
D1 Pas 1是一种小鼠常染色体死亡盒RNA解旋酶，主要在睾丸中表达。为了评估其可能的功能，我们使用具有靶向D1 Pas 1等位基因的胚胎干细胞产生D1 Pas 1缺陷小鼠。D1 Pas 1的缺失没有引起明显的胚胎缺陷或死亡，表明D1 Pas 1对胚胎发生不是必需的。而纯合子敲除雌性小鼠表现出正常的生殖性能，纯合子敲除雄性小鼠完全不育。D1 Pas 1缺陷的男性的生精上皮细胞中不含精子细胞或精子，因为在粗线期晚期的精子发生停滞。逆转录转座子如LINE-1的上调在D1 Pas 1缺陷的男性中没有发现，不像男性缺乏Mvh，另一种睾丸DEAD盒RNA解旋酶。D1 Pas 1缺陷的男性精母细胞减数分裂染色体的行为是难以区分的野生型男性，至少直到联会复合体的形成。因此，小鼠D1 Pas 1是第一个被鉴定的DEAD盒RNA解旋酶，在雄性生殖细胞第一次减数分裂前期的最后一步中发挥关键作用。(C)2016 Elsevier Inc. All rights reserved.
D1Pas1 is a mouse autosomal DEAD-box RNA helicase expressed predominantly in the testis. To assess its possible function, we generated D1Pasl-deficient mice using embryonic stem cells with a targeted D1Pas1 allele. Deletion of D1Pas1 did not cause noticeable embryonic defects or death, indicating that D1Pas1 is not essential for embryogenesis. Whereas homozygous knockout female mice showed normal reproductive performance, homozygous knockout male mice were completely sterile. The seminiferous epithelium of D1Pas1-deficient males contained no spermatids or spermatozoa because of spermatogenic arrest at the late pachytene stage. Upregulation of retrotransposons such as LINE-1 was not found in D1Pas1-deficient males, unlike males lacking Mvh, another testicular DEAD-box RNA helicase. Meiotic chromosome behavior in developing spermatocytes of D1Pas1-deficient males was indistinguishable from that in wild-type males, at least until synaptonemal complex formation. Thus, mouse D1Pas1 is the first-identified DEAD-box RNA helicase that plays critical roles in the final step of the first meiotic prophase in male germ cells. (C) 2016 Elsevier Inc. All rights reserved.