Meiotic behaviours of chromosomes and microtubules in budding yeast: relocalization of centromeres and telomeres during meiotic prophase
Meiotic behaviours of chromosomes and microtubules in budding yeast: relocalization of centromeres and telomeres during meiotic prophase
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DOI：
10.1046/j.1365-2443.1998.00215.x
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发表时间：
1998-09-01
期刊：
影响因子：
2.1
通讯作者：
Hiraoka, Y
中科院分区：
文献类型：
--
作者：
Hayashi, A;Ogawa, H;Hiraoka, Y
背景资料：减数分裂是真核生物中普遍重要的过程，通过染色体的重组和重新排列在可遗传的单倍体基因组中产生变异。染色体的核内运动是实现减数分裂过程中同源染色体的配对和重组，在芽殖酵母酿酒酵母减数分裂已被广泛研究，遗传学和分子生物学，在这里，我们报告减数分裂染色体事件在此organis.Results的细胞学观察：使用荧光显微镜，我们已经研究了染色体和微管在减数分裂过程中的行为在S。啤酒。我们首先观察了动态行为的细胞核在活细胞中使用水母绿色荧光蛋白（GFP）融合核质，非洲爪蟾卵母细胞核蛋白。通过对固定标本中的染色体和微管的分析，扩展了活细胞中核运动的特征。此外，在减数分裂细胞的同步群体中，通过间接免疫荧光显微镜确定了着丝粒和端粒的核定位。虽然端粒在整个减数分裂过程中保持为5-8个簇，但在减数分裂过程中，着丝粒的核定位发生了显着变化：在诱导减数分裂之前，着丝粒首先聚集在纺锤体附近的单个位点，并在减数分裂前期变得分散。结论：我们的观察表明，S.啤酒。特别是，在减数分裂过程中的着丝粒的独特的重新定位，表明在减数分裂前期细胞核内的染色体相当大的运动。
Background: Meiosis is a process of universal importance in eukaryotic organisms, generating variation in the heritable haploid genome by recombination and re-assortment of chromosomes. The intranuclear movement of chromosomes is expected to achieve pairing and recombination of homologous chromosomes during meiosis, Meiosis in the budding yeast Saccharomyces cerevisiae has been extensively studied, both genetically and by molecular biology; here we report cytological observations of meiotic chromosomal events in this organism.Results: Using fluorescence microscopy, we have examined the behaviour of chromosomes and microtubules during meiosis in S. cerevisiae. We first observed the dynamic behaviour of nuclei in living cells using jellyfish green fluorescent protein (GFP) fused with nucleoplasmin, a Xenopus oocyte nuclear protein. The characterization of nuclear movement in living cells was extended by an analysis of chromosomes and microtubules in fixed specimens. In addition, the nuclear localization of centromeres and telomeres was determined by indirect immunofluorescence microscopy in synchronous populations of meiotic cells. While telomeres remain in clusters of 5-8 throughout meiosis, centromeres change their nuclear localization dramatically during the progression of meiosis: centromeres are first clustered at a single site near the spindle-pole body before the induction of meiosis, and become scattered during the meiotic prophase.Conclusions: Our observations have demonstrated that nuclear and cytoskeletal reorganization take place with meiosis in S. cerevisiae. In particular, the distinct relocalization of centromeres during meiosis indicates a considerable movement of chromosomes within the meiotic prophase nucleus.