Microspatial variability in community structure and photophysiology of calcified macroalgal microbiomes revealed by coupling of hyperspectral and high-resolution fluorescence imaging.
Microspatial variability in community structure and photophysiology of calcified macroalgal microbiomes revealed by coupling of hyperspectral and high-resolution fluorescence imaging.
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DOI：
10.1038/srep22343
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发表时间：
2016-02-29
影响因子：
4.6
通讯作者：
Jesus B
中科院分区：
文献类型：
--
作者：
Perkins RG;Williamson CJ;Brodie J;Barillé L;Launeau P;Lavaud J;Yallop ML;Jesus B
钙化珊瑚巨藻为附生微藻提供了生物栖息地，对群落生产力有重要贡献。利用地理参考高光谱和高分辨率荧光成像技术，结合微空间映射的大藻寄主和附生微藻群落组成和相对生物量，确定了它们对岩岸上、下带officinalis寄主叶片生产力的加权贡献。下岸附生植物以丝状硅藻（硅藻门）为主，局限于叶状结构的顶端，适应弱光，但具有较高的光保护下调能力，贡献了群落总生产力的51%。上游附生植物以绿藻（绿藻）和单细胞硅藻（主要是球藻）为主，它们对光的适应程度较高，但相对生物量较低，对生产力的贡献可忽略不计。寄主officinalis是主要的初级生产者。由于海岸高度和宿主大型藻类的遮阳差异导致的光环境变化，可能在决定附生植物群落结构、生物量和生产力的模式中起着重要作用。此外，寄主大藻光生理参数的微空间梯度可能是由色素的年龄依赖性变化和光环境梯度引起的。
Calcifying coralline macroalgae provide biogenic habitats colonised by epiphytic microalgae that contribute significantly to community productivity. Georeferenced hyperspectral and high-resolution fluorescence imaging were coupled to microspatially mapped community composition and relative biomass of macroalgal host and epiphyte microalgal groups, and their weighted contributions to productivity within host fronds of Corallina officinalis on upper and lower zones of a rocky shore were determined. Lower shore epiphytes were dominated by filamentous diatoms (Bacillariophyta), confined to the apex of the frond structure, which were low light acclimated but retained a high capacity for photoprotective down regulation and contributed up to 51% of total community productivity. Upper shore epiphytes were dominated by green algae (Chlorophyta) and single-celled diatoms (principally Cocconeis spp.), which were high light acclimated but present at far lower relative biomass and contributed negligibly to productivity. The host, C. officinalis was the main primary producer. Variation in light environment resulting from differences in shore height and shading within the host macroalga, likely play a large role in determining patterns in epiphyte community structure, biomass and productivity observed. Additionally, microspatial gradients in photophysiological parameters along the host macroalga likely resulted from age-dependent variation in pigments as well as the gradient in light environment.