Binding and second messengers of prostaglandins F2 alpha and E1 in primary cultures of rabbit endometrial cells.
Binding and second messengers of prostaglandins F2 alpha and E1 in primary cultures of rabbit endometrial cells.
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兔子宫内膜细胞原代培养物中前列腺素 F2 α 和 E1 的结合和第二信使。
DOI：
10.1002/jcp.1041270109
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发表时间：
1986
影响因子：
5.6
通讯作者：
Gerschenson,LE
中科院分区：
文献类型：
--
作者：
Orlicky,DJ;Lieberman,R;Williams,C;Gerschenson,LE
一些因素和激素被认为在子宫内膜细胞的生长控制中起作用。我们已经证明前列腺素F2 α（PGF 2α）是在无血清、化学成分确定的培养基中生长的兔子宫内膜细胞的原代培养物的生长因子，并且前列腺素E1（PGE 1）拮抗PGF 2α对生长的诱导（Orlicky等人，1986年）。[3 H] PGF 2α与整个细胞的结合具有时间依赖性（最佳时间为30 min）和温度依赖性（最佳温度为37 ℃）、解离性（30 min内90%解离）、饱和性（Kd 1= 4.9× 10− 8 M，n 1= 1.2× 10 5分子/细胞; Kd 2= 2.6× 10− 7 M，n 2= 3.0× 10 5分子/细胞）和特异性。[3 H] PGE 1以时间依赖性（最佳25 min）、解离性（10 min内90%解离）、饱和性（Kd= 6.4× 10− 8 M，n= 1.2× 10 5分子/细胞）和特异性方式结合。[3 H] PGF 2α和[3 H] PGE 1的这种特异性结合可通过预先用未标记配体处理培养物而下调，并且可通过预先用吲哚美辛处理培养物以抑制内源性PG合成而上调。蛋白水解酶处理2 min可使PGF 2α的特异性结合降低75%。PGE 1以时间（最佳10 min）和浓度（10− 6 M时半数最大刺激）依赖性方式刺激细胞内cAMP合成和积累，但对细胞内cGMP无影响。PGF 2α对细胞内cAMP和cGMP均无影响。我们在这里描述的第一次在生物化学水平上的分析，相互拮抗的生长调节方面的两个arylandins之间的相互作用。
Several factors and hormones are thought to play a role in the growth control of endometrial cells. We have shown that prostaglandin F 2α (PGF 2α) is a growth factor for primary cultures of rabbit endometrial cells grown in serum-free, chemically defined medium and that prostaglandin E 1 (PGE 1) antagonizes the PGF 2α induction of growth (Orlicky et al., 1986).[3 H] PGF 2α binds to whole cells in a time (optimal∼ 30 min)-and temperature-dependent (optimal 37 C), disassociable (90% disassociable within 30 min), saturable (Kd 1= 4.9× 10− 8 M, n 1= 1.2× 10 5 molecules/cell; Kd 2= 2.6× 10− 7 M, n 2= 3.0× 10 5 molecules/cell), and specific manner.[3 H] PGE 1 binds in a time-dependent (optimal 25 min), disassociable (90% disassociable within 10 min), saturable (Kd= 6.4× 10− 8 M, n= 1.2× 10 5 molecules/cell), and specific manner. This specific binding of [3 H] PGF 2α and [3 H] PGE 1 is down-regulatable by prior treatment of the cultures with unlabeled ligand, and up-regulatable by prior treatment of the cultures with indomethacin to inhibit endogenous PG synthesis. Proteolytic enzyme treatment for 2 min reduces the specific binding of PGF 2α by 75%. PGE 1 stimulates intracellular cAMP synthesis and accumulation in a time (optimal 10 min)-and concentration (half-maximal stimulation at 10− 6 M)-dependent manner but has no effect on intracellular cGMP. PGF 2α has no effect on either intracellular cAMP or cGMP in this system. We describe here for the first time the analysis at a biochemical level of the interaction between two prostaglandins, antagonistic to each other in terms of growth regulation.