Detection of membrane receptors on per tumor cell by nonimmobilized cell capillary electrophoresis and a mathematic model
Detection of membrane receptors on per tumor cell by nonimmobilized cell capillary electrophoresis and a mathematic model
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通过非固定化细胞毛细管电泳和数学模型检测每个肿瘤细胞上的膜受体
DOI：
10.1016/j.talanta.2020.121425
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发表时间：
2021
期刊：
影响因子：
6.1
通讯作者：
Ling Xiaomei
中科院分区：
文献类型：
--
作者：
Wu Ruijun;Liang Chunsu;Zou Juncheng;Li Cong;Yan Youqi;Hu Xin;Ling Xiaomei
叶酸受体（FRs）是一类有价值的治疗靶点，在多种癌症中高表达。准确检测FR在不同细胞中的表达，有利于提高FR靶向肿瘤治疗的准确性。本文提出了一种基于非固定化细胞毛细管电泳（NICCE）和数学模型相结合的方法来定量每个肿瘤细胞上的fr。首先，我们通过NICCE分别研究了FA与A549、HT-29、HepG2和U87MG细胞的相互作用，并计算了动力学参数Ka、k′、Ka和kd。接下来，我们首次建立了准确测定A549、HT-29、HepG2和U87MG细胞上FRs的摩尔数的数学模型，分别为（10.44±0.53）× 10-19 mol、（34.32±1.33）× 10-19 mol、（337.14±10.11）× 10-19 mol和（37.31±2.13）× 10-19 mol。然后，这些结果与酶联免疫吸附试验（ELISA）结果一致。因此，该方法简便、快速、灵敏，无需蛋白分离纯化，有望实现临床对单个细胞上细胞膜受体表达水平的检测，对进一步的临床诊断和治疗大有裨益。
Folate receptors (FRs) are a class of valuable therapeutic target which is highly expressed on a variety of cancers. The accurate detection of the expression of FRs in different cells is conducive to improve the accuracy of FR targeted tumor therapy. Herein, a method based on nonimmobilized cell capillary electrophoresis (NICCE) combined with a mathematic model to quantify FRs on each single tumor cell was developed. At first, we studied the interactions between FA and A549, HT-29, HepG2, and U87MG cells by NICCE respectively, and calculated the kinetic parameters (Ka, k’, ka, and kd). Next, we established a mathematic model to accurately determine the number of moles of FRs on per A549, HT-29, HepG2, and U87MG cell for the first time, that were (10.44 ± 0.53) × 10–19 mol, (34.32 ± 1.33) × 10–19 mol, (337.14 ± 10.11) × 10–19 mol, and (37.31 ± 2.13) × 10–19 mol. Then, these re-sults were proved to be consistent with the results of enzyme-linked immunosorbent assay (ELISA). Therefore, this method is simple, rapid, sensitive, and without protein separation or purification, which is expected to achieve clinical detection of cell membrane receptor expression level of cell membrane receptors on a single cell, which may be greatly beneficial to further clinical diagnosis and therapy.