Characterization of glucan-producing Leuconostoc strains isolated from sourdough
Characterization of glucan-producing Leuconostoc strains isolated from sourdough
复制标题
DOI：
10.1016/j.ijfoodmicro.2010.05.026
复制
发表时间：
2010-11-15
影响因子：
5.4
通讯作者：
Fontagne-Faucher, Catherine
中科院分区：
文献类型：
--
作者：
Bounaix, Marie-Sophie;Gabriel, Valerie;Fontagne-Faucher, Catherine
酸面团之前被证明是一种富有成效的生境，可用于分离产生胞外多糖和更准确地说多样化的聚糖聚合物的乳酸菌，这些聚糖聚合物作为质地剂或益生元具有有趣的应用。通过 H-1 和 C-13 NMR 光谱分析对聚合物进行表征，表明这些菌株可以合成高度结构多样性的葡聚糖，并含有不同数量的 α-(1 -> 2)、α-(1 -> 3) 和 α-(1 -> 6) 键。在这项研究中，根据碳水化合物发酵、使用 (GTG)(5) 引物的rep-PCR 指纹分析和聚糖蔗糖酶活性（可溶性或细胞相关），对来自酸面团的 15 种产葡聚糖肠膜明串珠菌和柠檬酸柠檬菌株进行了表征。使用 SOS-PAGE 进行酶表征，以及与蔗糖孵育后的原位聚合物生产与经典或 α-(1 -> 2) 支链葡聚糖、奥特那和果聚糖的合成相关。此外，通过PCR检测编码交替蔗糖酶的基因的存在，并通过序列分析部分表征。因此，我们提供了有关酵母明串珠菌菌株生产葡聚糖的生物多样性的新信息。 (C) 2010 Elsevier B.V. 保留所有权利。
Sourdough was previously demonstrated to be a fruitful biotope for isolation of lactic acid bacteria producing exopolysaccharides and more accurately diverse glycan polymers which have interesting applications as texturing agents or prebiotics. Characterization of polymers by H-1 and C-13 NMR spectroscopy analysis demonstrated that these strains could synthesize glucans of high structural variety and containing different amounts of alpha-(1 -> 2), alpha-(1 -> 3) and alpha-(1 -> 6) linkages. In this study, fifteen glucan-producing Leuconostoc mesenteroides and L citreum strains from sourdoughs were characterized according to carbohydrate fermentation, rep-PCR fingerprinting using (GTG)(5) primers and glycansucrase activity (soluble or cell-associated). Enzyme characterization using SOS-PAGE and in situ polymer production after incubation with sucrose correlated with synthesis of classical or alpha-(1 -> 2) branched dextrans, alternan and levan. In addition, the presence of genes coding for alternansucrase was detected by PCR and partially characterized by sequence analysis. We thus provide new information on the biodiversity of glucan production by sourdough Leuconostoc strains. (C) 2010 Elsevier B.V. All rights reserved.