Zika virus in the Americas: Early epidemiological and genetic findings.
Zika virus in the Americas: Early epidemiological and genetic findings.
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DOI：
10.1126/science.aaf5036
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发表时间：
2016-04-15
期刊：
影响因子：
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通讯作者：
Vasconcelos PFC
中科院分区：
文献类型：
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作者：
Faria NR;Azevedo RDSDS;Kraemer MUG;Souza R;Cunha MS;Hill SC;Thézé J;Bonsall MB;Bowden TA;Rissanen I;Rocco IM;Nogueira JS;Maeda AY;Vasami FGDS;Macedo FLL;Suzuki A;Rodrigues SG;Cruz ACR;Nunes BT;Medeiros DBA;Rodrigues DSG;Queiroz ALN;da Silva EVP;Henriques DF;da Rosa EST;de Oliveira CS;Martins LC;Vasconcelos HB;Casseb LMN;Simith DB;Messina JP;Abade L;Lourenço J;Alcantara LCJ;de Lima MM;Giovanetti M;Hay SI;de Oliveira RS;Lemos PDS;de Oliveira LF;de Lima CPS;da Silva SP;de Vasconcelos JM;Franco L;Cardoso JF;Vianez-Júnior JLDSG;Mir D;Bello G;Delatorre E;Khan K;Creatore M;Coelho GE;de Oliveira WK;Tesh R;Pybus OG;Nunes MRT;Vasconcelos PFC
巴西经历了前所未有的寨卡病毒（ZIKV）流行，迄今已报告约30，000例病例。ZIKV于2015年5月首次在巴西被发现，2015年11月发现了可能与ZIKV感染相关的小头畸形病例。我们进行了下一代测序，生成了从四例自限性病例、一例献血者、一例致命成人病例和一例患有小头畸形和先天性畸形的新生儿中取样的七个巴西ZIKV基因组。系统发育和分子钟分析的结果显示ZIKV单次引入美洲，我们估计这发生在2013年5月至12月之间，比在巴西检测到ZIKV早12个月。估计的起源日期与从ZIKV流行地区前往巴西的航空乘客增加以及太平洋岛屿报告的疫情相吻合。来自巴西的ZIKV基因组与来自其他南美和加勒比国家的基因组在遗传学上散布。将突变映射到现有的结构模型上，揭示了爆发谱系中存在的病毒氨基酸变化的背景;然而，在来自小头畸形病例的三种目前可用的病毒基因组中没有发现共享的氨基酸变化。生育水平发病率数据表明，巴西疑似小头畸形的报告与妊娠第17周左右的ZIKV发病率最相关，尽管这种相关性并未证明因果关系。我们对巴西ZIKV分离株的遗传描述和分析为美洲这种新兴病毒的进化和分子流行病学的未来研究提供了基线。
Brazil has experienced an unprecedented epidemic of Zika virus (ZIKV), with ~30,000 cases reported to date. ZIKV was first detected in Brazil in May 2015, and cases of microcephaly potentially associated with ZIKV infection were identified in November 2015. We performed next-generation sequencing to generate seven Brazilian ZIKV genomes sampled from four self-limited cases, one blood donor, one fatal adult case, and one newborn with microcephaly and congenital malformations. Results of phylogenetic and molecular clock analyses show a single introduction of ZIKV into the Americas, which we estimated to have occurred between May and December 2013, more than 12 months before the detection of ZIKV in Brazil. The estimated date of origin coincides with an increase in air passengers to Brazil from ZIKV-endemic areas, as well as with reported outbreaks in the Pacific Islands. ZIKV genomes from Brazil are phylogenetically interspersed with those from other South American and Caribbean countries. Mapping mutations onto existing structural models revealed the context of viral amino acid changes present in the outbreak lineage; however, no shared amino acid changes were found among the three currently available virus genomes from microcephaly cases. Municipality-level incidence data indicate that reports of suspected microcephaly in Brazil best correlate with ZIKV incidence around week 17 of pregnancy, although this correlation does not demonstrate causation. Our genetic description and analysis of ZIKV isolates in Brazil provide a baseline for future studies of the evolution and molecular epidemiology of this emerging virus in the Americas.