High-Resolution Imaging of the Ocular Vasculature of Conjunctivitis in Mice Using Highly Bright Polymer Dots
High-Resolution Imaging of the Ocular Vasculature of Conjunctivitis in Mice Using Highly Bright Polymer Dots
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DOI：
10.1002/adhm.202200978
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发表时间：
2022-09-04
影响因子：
10
通讯作者：
Xiong，Liqin
中科院分区：
文献类型：
--
作者：
Li，Yuqiao;Lu，Shuting;Xiong，Liqin
眼部疾病主要由眼部血管畸变引起，对眼部血管结构的准确成像和分析至关重要。在本研究中，聚（9,9‐二辛烷基芴‐alt‐苯并噻唑）（PFBT）聚合物点（Pdots）具有易于合成、高亮度和低毒性的优点，被用作纳米探针，对眼球和视神经的血管系统进行高分辨率成像。此外，通过立体荧光显微镜对脉络膜微血管进行快速成像，分辨率高达1.6µm。结合组织清理和多光子显微技术，获得了视网膜主动脉和视神经微血管的全面三维血管信息。并比较结膜炎小鼠与正常小鼠的Schlemm管和虹膜血管密度。这些结果表明，PFBT Pdots在眼部疾病的快速、准确成像方面具有很大的应用潜力。
Ocular diseases are mainly caused by vascular aberrations in the eye, and accurate imaging and analysis of the ocular vascular structure is crucial. In this study, poly(9,9‐dioctylfluorene‐alt‐benzothiadiazole) (PFBT) polymer dots (Pdots), with the advantages of easy synthesis, high brightness, and low toxicity, are used as nanoprobes to perform high‐resolution imaging of the vasculature of the eyeball and optic nerve. Moreover, rapid imaging of the choroidal microvessels is carried out by stereoscopic fluorescence microscopy with a resolution of up to 1.6 µm. The comprehensive 3D vascular information of retinal aorta and optic nerve microvessels is obtained by combining tissue clearing and multiphoton microscopy. In addition, the vascular density of Schlemm's canal and iris blood vessels is compared between the conjunctivitis mice and the normal mice. These results suggest that PFBT Pdots have great application potential in the fast and accurate imaging of ocular diseases.