Relationships between plasma lipidomic profiles and brown adipose tissue density in humans
Relationships between plasma lipidomic profiles and brown adipose tissue density in humans
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DOI：
10.1038/s41366-020-0558-y
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发表时间：
2020-03-03
影响因子：
4.9
通讯作者：
Hamaoka, Takafumi
中科院分区：
文献类型：
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作者：
Fuse, Sayuri;Sugimoto, Masahiro;Hamaoka, Takafumi
背景/目的棕色脂肪组织（brown aditissue， BAT）的产热功能通常在冬季被激活，并受到多种代谢过程的严格调控。然而，介导这些变化的机制尚未在人类中阐明。在这里，我们研究了冬季和夏季男性和女性血浆中BAT密度（BAT-d）与脂质代谢物之间的关系。受试者/方法共采集血浆样本92份，分别来自23名男性和23名女性，年龄21-55岁，分别在夏季和冬季采集。脂质代谢物采用液相色谱-飞行时间质谱法全面定量。采用近红外时间分辨光谱法测定锁骨上区总血红蛋白浓度来评价BAT-d。人体测量参数，如全身脂肪百分比和内脏脂肪面积（VFA）进行评估。采用单因素和多因素回归分析探讨影响BAT-d的因素。结果检测到甘油磷脂（168个峰）、甾体及其衍生物（78个峰）、脂肪酰基（62个峰）、甘油脂（31个峰）等多种代谢物峰。单因素回归分析，通过错误发现率修正得出Q值，发现夏季男性的batd与磷脂酰乙醇胺(PE（46:2), r = 0.62, Q = 4.9 × 10(-2)）、冬季的雄激素（r = 0.75, Q = 7.0 × 10(-3)）、夏季的二酰基甘油(DG（36:1), r = -0.68, Q = 4.9 × 10(-2)）有显著相关性，而女性无显著相关性。冬季多因素回归分析显示，男性中BAT-d与血浆雄激素（P = 5.3 × 10(-5)）之间存在显著相关性，女性中BAT-d与VFA （P = 2.2 × 10(-3)）之间存在显著相关性。结论血浆中某些脂质与BAT-d的相关性与性别和季节有关。在冬季，BAT-d显示出与男性血浆雄激素的特殊相关性。
Background/objectives The thermogenic function of brown adipose tissue (BAT) is generally activated in winter and tightly regulated through various metabolic processes. However, the mechanisms mediating these changes have not been elucidated in humans. Here, we investigated the relationships between BAT density (BAT-d) and lipid metabolites in plasma from men and women in the winter and summer. Subjects/methods In total, 92 plasma samples were obtained from 23 men and 23 women, aged 21-55 years, on two different occasions (summer and winter). Lipid metabolites were comprehensively quantified using liquid chromatography-time-of-flight-mass spectrometry. BAT-d was evaluated by measuring total hemoglobin concentrations in the supraclavicular region using near-infrared time-resolved spectroscopy. Anthropometric parameters, such as the percentage of whole body fat and visceral fat area (VFA), were evaluated. Factors influencing BAT-d were investigated by univariate and multivariate regression analyses. Results A variety of metabolite peaks, such as glycerophospholipids (168 peaks), steroids and derivatives (78 peaks), fatty acyls (62 peaks), and glycerolipids (31 peaks), were detected. Univariate regression analysis, corrected by false discovery rate to yield Q values, revealed significant correlations in BAT-d and phosphatidylethanolamine (PE(46:2), r = 0.62, Q = 4.9 x 10(-2)) in the summer, androgens (r = 0.75, Q = 7.0 x 10(-3)) in the winter, and diacylglycerol (DG(36:1), r = -0.68, Q = 4.9 x 10(-2)) in the summer in men, but not in women. Multivariate regression analysis in the winter revealed a significant correlation between BAT-d and plasma androgens (P = 5.3 x 10(-5)) in men and between BAT-d and VFA (P = 2.2 x 10(-3)) in women. Conclusions Certain lipids in plasma showed unique correlations with BAT-d depending on sex and season. BAT-d showed a specific correlation with plasma androgens in men in the winter.