Dendrimer-entrapped gold nanoparticles as a platform for cancer-cell targeting and Imaging
Dendrimer-entrapped gold nanoparticles as a platform for cancer-cell targeting and Imaging
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DOI：
10.1002/smll.200700054
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发表时间：
2007-07-01
期刊：
影响因子：
13.3
通讯作者：
Baker, James R., Jr.
中科院分区：
文献类型：
--
作者：
Shi, Xiangyang;Wang, Suhe;Baker, James R., Jr.
我们提出了一种使用树枝状大分子包埋的金纳米颗粒（所有 DENP）对癌细胞进行靶向和成像的通用方法。人们发现 Au DENP 能够与靶向和成像配体共价连接，用于随后的癌细胞靶向和成像。 Au DENP 与规定数量的叶酸 (FA) 和异硫氰酸荧光素 (H) 分子连接，具有水溶性、稳定且生物相容性。体外研究表明，FA 和 FI 修饰的 Au DENP 可以与过度表达高亲和力叶酸受体的 KB 细胞（人上皮癌细胞系）特异性结合，并在 2 小时内主要内化到靶细胞的溶酶体中。这些发现表明 Au DENP 可以作为癌症成像和治疗的通用平台。
We present a general approach for the targeting and imaging of cancer cells using dendrimer-entrapped gold nanoparticles (All DENPs). Au DENPs were found to be able to covalently link with targeting and imaging ligands for subsequent cancer-cell targeting and imaging. The Au DENPs linked with defined numbers of folic acid (FA) and fluorescein isothiocyanate (H) molecules are water soluble, stable, and biocompatible. In vitro studies show that the FA- and FI-modified Au DENPs can specifically bind to KB cells (a human epithelial carcinoma cell line) that overexpress high-affinity folate receptors and they are internalized dominantly into lysosomes of target cells within 2 h. These findings demonstrate that Au DENPs may serve as a general platform for cancer imaging and therapeutics.